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Updated: May 1, 2026

Detection of G Protein-coupled Receptor Expression in Mouse Vagal Afferent Neurons using Multiplex In Situ Hybridization
Published on: September 20, 2021
Five novel variants of GPR103 and their expression in different tissues of goose (Anser cygnoides)
Qihai Xiao1, Dandan Liu1, Liang Li1
1Institute of Animal Breeding & Genetics, Sichuan Agricultural University, Ya'an, Sichuan 625014, PR China.
Abstract:
GPR103 plays an important role in various tissues, while little information is available about the alternative splicing (AS) of its mRNA. In the present study, we used genomic PCR to identify the partial genomic locus of goose (Anser cygnoides) GPR103 and rapid amplification of cDNA ends (RACE)-PCR to identify five GPR103 variants, including the full-length variant (aGPR103-n) and four alternatively spliced variants (aGPR103-va, -vb, -vc and -vd). Sequence analysis showed that aGPR103-va and -vd are less likely to undergo nonsense-mediated mRNA decay, suggesting that they may be translated into truncated proteins. Quantitative real-time PCR (qRT-PCR) analysis revealed that the five variants are widely distributed in the brain and peripheral tissues of geese and show specific expression patterns. Thus, we here provide the first account of the GPR103 genomic locus and illustrate its transcriptional diversity and widespread distribution in geese.
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