Analysis of thiopurines using aqueous normal phase chromatography
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Updated: May 1, 2026

Synthesis and Purification of Iodoaziridines Involving Quantitative Selection of the Optimal Stationary Phase for Chromatography
Published on: May 16, 2014
Joseph J Pesek1, Maria T Matyska1, Joshua E Young2
1Department of Chemistry, San Jose State University, San Jose, CA 95112, USA.
Abstract:
The chromatography of several thiopurines is investigated using aqueous normal phase (ANP) conditions in conjunction with a silica hydride-based column. Both isocratic and gradient elution modes are tested. Detection of higher concentration samples is done by UV to demonstrate feasibility in this format while lower concentration samples utilize mass spectrometry (MS). Repeatability of successive runs is also tested with particular attention to gradient methods where the equilibration time of the stationary phase can be evaluated.
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