Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Notch Signaling Pathway03:14

Notch Signaling Pathway

4.6K
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
4.6K
Notch Signaling Pathway03:14

Notch Signaling Pathway

4.9K
4.9K
Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

1.8K
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
1.8K
Development of the Lymphatic System01:15

Development of the Lymphatic System

2.8K
The development of lymphatic tissues and vessels in embryonic life begins around the fifth week. These structures originate from the mesoderm layer, with lymph sacs emerging from developing veins.
The first lymph sacs to form are the paired jugular lymph sacs located at the junction of the internal jugular and subclavian veins. From these sacs, lymphatic capillary plexuses extend to the thorax, upper limbs, neck, and head, eventually forming lymphatic vessels. Each jugular lymph sac maintains a...
2.8K
Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

2.9K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
2.9K
Determination01:51

Determination

16.3K
During embryogenesis, cells become progressively committed to different fates through a two-step process: specification followed by determination. Specification is demonstrated by removing a segment of an early embryo, “neutrally” culturing the tissue in vitro—for example, in a petri dish with simple medium—and then observing the derivatives. If the cultured region gives rise to cell types that it would normally generate in the embryo, this means that it is specified. In...
16.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

AXIN1 and AXIN2 regulate the WNT-signaling landscape to promote distinct mesoderm programs.

Developmental cell·2026
Same author

Constitutive YAP activation in distal nephron segments disrupts epithelial identity and nephron patterning.

JCI insight·2026
Same author

L1CAM signaling through planar cell polarity drives SOX2 expression and lung adenocarcinoma metastasis.

Nature communications·2026
Same author

Asymmetric attrition and secondary chromosome destabilization after double-strand breaks in human embryonic development.

Nature communications·2026
Same author

Transcription factor 21 deletion from podocyte precursors as a model for congenital nephrotic syndrome.

American journal of physiology. Renal physiology·2026
Same author

PROX1 loss in adult mouse Schlemm's canal causes permanent ocular hypertension.

JCI insight·2026

Related Experiment Video

Updated: May 1, 2026

En Face Endocardial Cushion Preparation for Planar Morphogenesis Analysis in Mouse Embryos
08:57

En Face Endocardial Cushion Preparation for Planar Morphogenesis Analysis in Mouse Embryos

Published on: July 27, 2022

1.2K

Murine Notch1 is required for lymphatic vascular morphogenesis during development.

Anees Fatima1, Austin Culver, Ford Culver

  • 1Feinberg Cardiovascular Research Institute, Feinberg School of Medicine, Northwestern University, Chicago, Illinois.

Developmental Dynamics : an Official Publication of the American Association of Anatomists
|March 25, 2014
PubMed
Summary

Notch1 signaling in lymphatic endothelial cells inhibits developmental lymphangiogenesis, preventing excessive lymphatic vessel growth. Loss of Notch1 leads to enlarged lymphatic vessels due to increased sprouting and reduced cell death.

Keywords:
NotchProx1lymphangiogenesislymphatic vessel development

More Related Videos

A Method for Labeling Vasculature in Embryonic Mice
09:58

A Method for Labeling Vasculature in Embryonic Mice

Published on: October 7, 2011

15.4K
Stimulation of Notch Signaling in Mouse Osteoclast Precursors
08:01

Stimulation of Notch Signaling in Mouse Osteoclast Precursors

Published on: February 28, 2017

7.7K

Related Experiment Videos

Last Updated: May 1, 2026

En Face Endocardial Cushion Preparation for Planar Morphogenesis Analysis in Mouse Embryos
08:57

En Face Endocardial Cushion Preparation for Planar Morphogenesis Analysis in Mouse Embryos

Published on: July 27, 2022

1.2K
A Method for Labeling Vasculature in Embryonic Mice
09:58

A Method for Labeling Vasculature in Embryonic Mice

Published on: October 7, 2011

15.4K
Stimulation of Notch Signaling in Mouse Osteoclast Precursors
08:01

Stimulation of Notch Signaling in Mouse Osteoclast Precursors

Published on: February 28, 2017

7.7K

Area of Science:

  • Developmental Biology
  • Vascular Biology
  • Molecular Signaling

Background:

  • The Notch1 receptor is crucial for blood vessel development.
  • Previous studies indicated Notch1 and Dll4 blockade cause postnatal lymphatic defects.
  • The specific role of Notch signaling in lymphatic development remained unclear.

Purpose of the Study:

  • To elucidate the developmental role of Notch1 in lymphatic vascular morphogenesis.
  • To investigate the function of Notch signaling in lymphatic endothelial cells (LECs) during development.

Main Methods:

  • Generation of LEC-specific conditional Notch1 knockout mice using Prox1CreER(T2) driver.
  • Analysis of lymphatic vessel development in mutant embryos.
  • RNA-sequencing (RNA-seq) to assess gene expression changes in mutant LECs.

Main Results:

  • LEC-specific Notch1 deficiency resulted in enlarged lymphatic vessels and increased LEC sprouting.
  • Notch1-mutant LECs showed increased filopodia formation, reduced cell death, and increased proliferation.
  • RNA-seq revealed upregulation of cytokine/chemokine signaling and downregulation of VEGFR3, VEGFR2, VEGFC, and Gja4 in mutant LECs.

Conclusions:

  • Notch activity within LECs is essential for controlling lymphatic sprouting and growth during development.
  • The Notch signaling pathway acts as an inhibitor of developmental lymphangiogenesis.
  • These findings extend the known role of Notch signaling in lymphangiogenesis to the developmental stage.