Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

DNA methylation and differentiation.

L A Michalowsky1, P A Jones

  • 1Department of Biochemistry, USC Cancer Center, Los Angeles 90033.

Environmental Health Perspectives
|March 1, 1989
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comprehensive Lifestyle Improvement Program for Prostate Cancer (CLIPP) is associated with improvement in weight and components of metabolic syndrome in men exposed to androgen deprivation therapy for prostate cancer.

Prostate cancer and prostatic diseases·2021
Same author

DNA methylation in blood from neonatal screening cards and the association with BMI and insulin sensitivity in early childhood.

International journal of obesity (2005)·2017
Same author

Pharmacokinetics and toxicity of the novel oral demethylating agent zebularine in laboratory and tumor bearing dogs.

Veterinary and comparative oncology·2015
Same author

NMDA receptor binding in focal epilepsies.

Journal of neurology, neurosurgery, and psychiatry·2015
Same author

Pulsed-coil magnet systems for applying uniform 10-30 T fields to centimeter-scale targets on Sandia's Z facility.

The Review of scientific instruments·2015
Same author

Development of the quiescent center in maturing embryonic radicles of pea (Pisum sativum L. cv. Alaska).

Planta·2014
Same journal

A New Start.

Environmental health perspectives·2026
Same journal

Time-Varying Exposure to Element Mixtures and Children's Cognition at 5 Years of Age: Findings from the New Hampshire Birth Cohort Study.

Environmental health perspectives·2026
Same journal

Effect of Household Air Pollution on the Gut Microbiome and Virome of Adult Women Living in Uganda.

Environmental health perspectives·2026
Same journal

Comparison of Temperature-Mortality Associations across the Middle East Using Different Exposure Estimation Approaches.

Environmental health perspectives·2026
Same journal

Workflow for Statistical Analysis of Environmental Mixtures.

Environmental health perspectives·2026
Same journal

Effects of Extreme Heat Exposure on Heatstroke and Liver Injury in Mice: The Role of PPARα.

Environmental health perspectives·2026
See all related articles

DNA methylation regulates gene expression and cell function. Inhibiting DNA methylation with 5-azacytidine alters cell phenotypes by affecting gene activation and imprinting.

Area of Science:

  • Epigenetics
  • Molecular Biology
  • Genetics

Background:

  • DNA methylation, specifically at cytosine residues, is crucial for gene regulation and cellular specialization.
  • Demethylation of CpG sites often correlates with increased gene transcription.
  • Cytosine methylation is vital for parental imprinting, controlling differential gene expression from maternal and paternal genomes during embryogenesis.

Purpose of the Study:

  • To explore the role of DNA methylation in gene expression and cellular phenotype.
  • To investigate the effects of 5-azacytidine, a DNA methylation inhibitor, on cellular processes.
  • To understand how DNA methylation patterns are maintained during cell division.

Main Methods:

  • Utilized 5-azacytidine to inhibit DNA methylation in cellular models.

Related Experiment Videos

  • Observed phenotypic alterations following drug treatment.
  • Examined the impact of hypomethylation on DNA-protein interactions and chromatin structure.

    • Main Results:

    • Treatment with 5-azacytidine induced significant phenotypic changes.
    • Drug-induced DNA hypomethylation disrupted DNA-protein interactions, affecting transcriptional activity and cell determination.
    • Methylation patterns were shown to be stable and heritable, acting as a cellular memory mechanism.

    Conclusions:

    • DNA methylation is a key regulator of gene expression, cellular phenotype, and genomic imprinting.
    • Inhibiting DNA methylation can lead to profound cellular alterations by perturbing gene regulation and epigenetic memory.
    • Methylation patterns serve as a heritable memory, influencing cellular identity across cell divisions.