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Simultaneous sequencing of RNA by solid-phase chemical degradation using DE 81 anion-exchange paper
1Akademie der Wissenschaften der DDR.
Gene Analysis Techniques
|May 1, 1987
Abstract:
A solid-phase chemical degradation method for simultaneous sequencing of RNA and RNA fragments has been developed using Whatman DE 81 anion-exchange paper as the support. The approach involves the following operations: (1) immobilization of the 3'-end labeled RNAs or RNA fragments on DE 81 paper; (2) washing; (3) modification reactions; (4) washing; (5) sorting of the paper segments; (6) aniline reaction; (7) lyophilization; (8) desorption of the RNA by TEAB; and (9) lyophilization.