Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Bioreactor Controls-I01:28

Bioreactor Controls-I

94
Maintaining optimal conditions within fermenters is essential for maximizing microbial productivity and ensuring process efficiency. This lesson focuses on key parameters—temperature, foam, pH, carbon dioxide, oxygen, and pressure—and their precise measurement and control strategies in fermentation systems.Temperature ControlTemperature regulation is critical due to the exothermic nature of many fermentation processes. In small laboratory fermenters, temperature is commonly...
94
Bioreactor Controls-II01:18

Bioreactor Controls-II

76
In aerobic fermentations, oxygen is vital for microbial growth and metabolite production. Since air comprises only about 20% oxygen and the gas is poorly soluble in water—just 9 ppm at 20°C—supplying sufficient oxygen becomes a critical challenge, especially in high-demand processes like yeast growth or citric acid production. Even a fully saturated broth may offer only a few seconds of oxygen availability.To address this, sterile or scrubbed air is introduced into the...
76
Bioreactor Controls-III01:22

Bioreactor Controls-III

67
Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...
67
Bioreactor Design and Operational System01:29

Bioreactor Design and Operational System

200
Bioreactors are engineered vessels designed to cultivate microorganisms under controlled conditions for industrial bioprocessing. They maintain sterility and allow precise regulation of pH, temperature, oxygen, and nutrient levels to optimize microbial growth and metabolite production. Bioreactors range from small laboratory units of 1 liter to industrial systems holding up to 500,000 liters, though only about 75% of their volume is actively used for fermentation. The remaining headspace...
200
Designing Growth Media for Bioreactors01:30

Designing Growth Media for Bioreactors

78
Growth media provide essential nutrients that support cell growth and metabolism, thereby enhancing the yield of valuable products such as enzymes, antibiotics, and biomass. Designing an effective growth medium involves balancing all components to prevent nutrient limitations or toxic excesses, both of which can impair growth and reduce product yields.Composition of a Typical Growth MediumA typical growth medium contains carbon and nitrogen sources, salts, vitamins, trace elements, and...
78
Upstream Processing01:27

Upstream Processing

97
Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...
97

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Drainage from a Fluid-Handling Component Due to Centrifugal Acceleration.

Langmuir : the ACS journal of surfaces and colloids·2018
Same author

Thermodynamic Constraints Improve Metabolic Networks.

Biophysical journal·2017
Same author

Stringent control of FLP recombinase in Escherichia coli.

Journal of microbiological methods·2016
Same author

Unsupervised Identification of Isotope-Labeled Peptides.

Analytical chemistry·2016
Same author

Sequence-based Network Completion Reveals the Integrality of Missing Reactions in Metabolic Networks.

The Journal of biological chemistry·2015
Same author

Genome-scale metabolic network validation of Shewanella oneidensis using transposon insertion frequency analysis.

PLoS computational biology·2014

Related Experiment Video

Updated: May 1, 2026

Operation of a Benchtop Bioreactor
12:54

Operation of a Benchtop Bioreactor

Published on: September 12, 2013

93.4K

Open source software to control Bioflo bioreactors.

David A Burdge1, Igor G L Libourel2

  • 1Biotechnology Institute, University of Minnesota, Saint Paul, Minnesota, United States of America.

Plos One
|March 27, 2014
PubMed
Summary

This study introduces flexible, open-source freeware for controlling Bioflo bioreactors, enabling custom experiments and auxiliary hardware integration without expensive proprietary software.

More Related Videos

Use of a High-throughput In Vitro Microfluidic System to Develop Oral Multi-species Biofilms
07:09

Use of a High-throughput In Vitro Microfluidic System to Develop Oral Multi-species Biofilms

Published on: December 1, 2014

12.5K
Design and Implementation of an Automated Illuminating, Culturing, and Sampling System for Microbial Optogenetic Applications
11:13

Design and Implementation of an Automated Illuminating, Culturing, and Sampling System for Microbial Optogenetic Applications

Published on: February 19, 2017

9.4K

Related Experiment Videos

Last Updated: May 1, 2026

Operation of a Benchtop Bioreactor
12:54

Operation of a Benchtop Bioreactor

Published on: September 12, 2013

93.4K
Use of a High-throughput In Vitro Microfluidic System to Develop Oral Multi-species Biofilms
07:09

Use of a High-throughput In Vitro Microfluidic System to Develop Oral Multi-species Biofilms

Published on: December 1, 2014

12.5K
Design and Implementation of an Automated Illuminating, Culturing, and Sampling System for Microbial Optogenetic Applications
11:13

Design and Implementation of an Automated Illuminating, Culturing, and Sampling System for Microbial Optogenetic Applications

Published on: February 19, 2017

9.4K

Area of Science:

  • Biotechnology
  • Software Engineering

Background:

  • Commercially available bioreactors offer controlled environments but often lack software flexibility for custom experiments or integrating auxiliary hardware.
  • Existing bioreactor software may not support novel experimental designs or third-party hardware, necessitating custom software development.

Purpose of the Study:

  • To develop a flexible, open-source freeware solution for controlling Bioflo bioreactors.
  • To enable custom experimental protocols and seamless integration of auxiliary hardware.
  • To provide a cost-effective and adaptable alternative to proprietary bioreactor control software.

Main Methods:

  • Development of open-source freeware with setpoint control, data logging, and protocol execution capabilities.
  • Implementation of a plugin interface for easy integration and control of auxiliary hardware.
  • Inclusion of CSV scripting for simple protocols and a Python-based model for complex, conditional experimental control.

Main Results:

  • The developed software provides flexible control for Bioflo bioreactors, including advanced features like conditional protocol execution.
  • An integrated plugin interface allows for straightforward addition and control of custom or third-party auxiliary hardware.
  • The software is available as a self-installing package for Windows, eliminating the need for LabVIEW.

Conclusions:

  • The open-source freeware offers a versatile and free alternative to commercial bioreactor control solutions.
  • The software enhances experimental capabilities by supporting custom protocols and auxiliary hardware integration.
  • Public availability of source code and plugins promotes community-driven development and broader adoption in research settings.