Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Circular Bivalent Aptamer Chimeras Leveraging LDLR-Mediated Lysosomal Shuttling for Targeted Protein Degradation.

Journal of medicinal chemistry·2026
Same author

Antegrade submental artery osteomyocutaneous flap for the repair of maxillary alveolar process defects combined with personalized 3D-printed PEEK material for restoration of mandibular inferior border defects.

BMC oral health·2026
Same author

Spectral decoupling between radioluminescence and persistent luminescence in Bi<sup>3+</sup>-doped LiLu(WO<sub>4</sub>)<sub>2</sub> for high-security information encryption.

Chemical communications (Cambridge, England)·2026
Same author

Calcium-rich biochar/lanthanum alginate hydrogel beads for fluoride removal from groundwater.

International journal of biological macromolecules·2026
Same author

Aptamer-Mediated Proximity Labeling (ApMPL) for Spatially Resolved Deciphering of Cell Membrane Protein Interactomes.

Analytical chemistry·2026
Same author

Theoretical Dynamics Modeling of Pitch Motion and Obstacle-Crossing Capability Analysis for Articulated Tracked Vehicles Based on Myriapod Locomotion Mechanism.

Biomimetics (Basel, Switzerland)·2026

Related Experiment Video

Updated: May 1, 2026

Author Spotlight: Enhancing Bone Regeneration with Vascularized Artificial Cartilage Integration
06:05

Author Spotlight: Enhancing Bone Regeneration with Vascularized Artificial Cartilage Integration

Published on: July 14, 2023

1.8K

Construction of functional tissue-engineered bone using cell sheet technology in a canine model.

Tao Chen1, Yanhui Wang1, Lingxue Bu1

  • 1Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Qingdao University Medical College, Qingdao, Shandong 266003, P.R. China.

Experimental and Therapeutic Medicine
|March 27, 2014
PubMed
Summary

Cell sheet technology using bone mesenchymal stem cells (BMSCs) effectively constructs functional tissue-engineered bone. This innovative method significantly enhances osteogenesis compared to traditional techniques, promoting ideal bone regeneration.

Keywords:
cell sheet technologymesenchymal stem cellstissue-engineered bone

More Related Videos

Author Spotlight: Insights into the Use of Apple-Derived Cellulose Scaffolds for Bone Tissue Engineering
09:49

Author Spotlight: Insights into the Use of Apple-Derived Cellulose Scaffolds for Bone Tissue Engineering

Published on: February 23, 2024

2.8K
Use of Human Perivascular Stem Cells for Bone Regeneration
07:05

Use of Human Perivascular Stem Cells for Bone Regeneration

Published on: May 25, 2012

23.2K

Related Experiment Videos

Last Updated: May 1, 2026

Author Spotlight: Enhancing Bone Regeneration with Vascularized Artificial Cartilage Integration
06:05

Author Spotlight: Enhancing Bone Regeneration with Vascularized Artificial Cartilage Integration

Published on: July 14, 2023

1.8K
Author Spotlight: Insights into the Use of Apple-Derived Cellulose Scaffolds for Bone Tissue Engineering
09:49

Author Spotlight: Insights into the Use of Apple-Derived Cellulose Scaffolds for Bone Tissue Engineering

Published on: February 23, 2024

2.8K
Use of Human Perivascular Stem Cells for Bone Regeneration
07:05

Use of Human Perivascular Stem Cells for Bone Regeneration

Published on: May 25, 2012

23.2K

Area of Science:

  • Regenerative Medicine
  • Biomaterials Science
  • Orthopedic Surgery

Background:

  • Traditional bone tissue engineering faces challenges in achieving optimal osteogenesis and vascularization.
  • Cell sheet technology offers a promising approach for creating functional tissue constructs by preserving cell-cell interactions and extracellular matrix.
  • Bone mesenchymal stem cells (BMSCs) are crucial for bone regeneration due to their osteogenic potential.

Purpose of the Study:

  • To construct functional tissue-engineered bone using canine bone mesenchymal stem cells (BMSCs) via cell sheet technology.
  • To compare the efficacy of BMSC cell sheet-based bone regeneration with traditional bone tissue engineering methods.
  • To evaluate the impact of cell sheet technology on growth factor expression and osteogenesis in vivo.

Main Methods:

  • Canine BMSCs were isolated, induced into osteoblasts, and cultured on temperature-responsive dishes to form cell sheets.
  • A demineralized bone matrix (DBM)/platelet-rich plasma (PRP)/BMSC cell sheet complex was constructed and implanted in a canine model.
  • A control group received a DBM/PRP/BMSC complex without cell sheets; growth factor levels and osteogenesis were assessed.

Main Results:

  • The BMSC cell sheet implants demonstrated significantly enhanced osteogenesis compared to the control group.
  • Expression levels of platelet-derived growth factor (PDGF) and vascular endothelial growth factor (VEGF) were significantly higher (3.2- and 2.5-fold, respectively) in the cell sheet group.
  • The BMSC cell sheets were confirmed to be functional and superior to the traditional cell complex for bone regeneration.

Conclusions:

  • Cell sheet technology using BMSCs is an effective strategy for constructing functional tissue-engineered bone.
  • This approach promotes superior osteogenesis and increased growth factor expression, leading to improved bone regeneration.
  • BMSC cell sheet technology holds significant potential for developing advanced bone regenerative therapies.