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Screening of Caatinga plants as sources of lectins and trypsin inhibitors
José Hélton Vasconcelos Arcoverde1, Aline de Souza Carvalho, Fernanda Pacífico de Almeida Neves
1a Departamento de Bioquímica , CCB, Universidade Federal de Pernambuco, Cidade Universitária , 50670-420 Recife , Pernambuco , Brazil.
Abstract:
Although it is one of the most threatened areas in the Earth, there are few studies on the biotechnological potential of the Caatinga. This work evaluated 36 extracts from 27 Caatinga plants for lectin and trypsin inhibitor activities. The presence of lectin was detected in 77.7% of samples by haemagglutinating assay. The highest values of specific haemagglutinating activity were found in extracts of leaves from Mimosa lewesii, Bauhinia acuruana and Manilkara rufula and in branches from Myracrodruon urundeuva. Trypsin inhibitor activity was detected in 63.9% of the tested extracts, strong inhibitory effect (>70%) being found in 11 samples. This work demonstrates that Caatinga is a potential source of bioactive plant proteins that can be isolated and studied for several applications. The biochemical prospecting of Caatinga is essential for collection of bioactive principles so as to add conservation value to the region.
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