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Progressive-ratio Responding for Palatable High-fat and High-sugar Food in Mice

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An acute method to test leptin responsiveness in rats.

Bhavna N Desai¹, Ruth B S Harris²

¹Department of Physiology, Medical College of Georgia, Georgia Regents University, Augusta, Georgia bdesai@gru.edu.

American Journal of Physiology. Regulatory, Integrative and Comparative Physiology

|March 28, 2014

Summary

An acute intravenous leptin infusion effectively lowered blood glucose in leptin-responsive rats, establishing a novel method for assessing leptin sensitivity in rodent models.

Keywords:

blood glucose leptin infusion leptin resistance

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Area of Science:

Endocrinology
Metabolic Research
Pharmacology

Background:

Leptin administration normalizes blood glucose in diabetic rodents, independent of metabolic changes.
Leptin resistance is a significant factor in metabolic disorders.
Assessing leptin sensitivity is crucial for understanding its therapeutic potential.

Purpose of the Study:

To determine if acute intravenous leptin infusion can serve as a marker for leptin sensitivity.
To investigate the glucose-lowering effects of leptin in normal and leptin-resistant rats.

Main Methods:

Rats (leptin-responsive and diet-induced leptin-resistant) received intravenous infusions of saline, low-dose (LD) leptin followed by high-dose (HD) leptin, or only HD leptin.
Blood glucose, serum leptin, and plasma glucagon levels were monitored.
Food intake and body weight were also assessed.

Main Results:

Combined LD and HD leptin infusion significantly decreased serum glucose in leptin-responsive rats but not in leptin-resistant rats.
Leptin infusions increased serum leptin in all groups without affecting glucagon, weight gain, or energy intake.
No significant glucose changes were observed with LD or HD leptin alone.

Conclusions:

Leptin exhibits an acute glucose-lowering effect that directly correlates with leptin responsiveness in rats.
This acute intravenous infusion test offers a novel, nonlethal method for evaluating leptin sensitivity across different rat models.