Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

1.9K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
1.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Membrane-associated effluxosomes coordinate multi-metal resistance in Mycobacterium tuberculosis.

The EMBO journal·2026
Same author

Fast volumetric fluorescence lifetime imaging of multicellular systems using single-objective light-sheet microscopy.

Communications biology·2025
Same author

In-depth single molecule localization microscopy using adaptive optics and single objective light-sheet microscopy.

Nature communications·2025
Same author

PacL-organized membrane-associated effluxosomes coordinate multi-metal resistance in <i>Mycobacterium tuberculosis</i>.

bioRxiv : the preprint server for biology·2025
Same author

Author Correction: Arkitekt: streaming analysis and real-time workflows for microscopy.

Nature methods·2025
Same author

Arkitekt: streaming analysis and real-time workflows for microscopy.

Nature methods·2024

Related Experiment Video

Updated: May 1, 2026

A Protocol for Real-time 3D Single Particle Tracking
10:16

A Protocol for Real-time 3D Single Particle Tracking

Published on: January 3, 2018

14.5K

High-density single-particle tracking: quantifying molecule organization and dynamics at the nanoscale.

Jean-Baptiste Sibarita1

  • 1Interdisciplinary Institute for Neuroscience, CNRS UMR 5297, University of Bordeaux, 33000, Bordeaux, France, jean-baptiste.sibarita@u-bordeaux2.fr.

Histochemistry and Cell Biology
|March 28, 2014
PubMed
Summary

Single-particle tracking (SPT) microscopy visualizes protein organization and dynamics. High-density SPT advances this, enabling detailed cellular compound analysis with improved statistics.

More Related Videos

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules
10:20

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Published on: September 5, 2019

7.9K
Mass-Sensitive Particle Tracking to Characterize Membrane-Associated Macromolecule Dynamics
13:30

Mass-Sensitive Particle Tracking to Characterize Membrane-Associated Macromolecule Dynamics

Published on: February 18, 2022

7.7K

Related Experiment Videos

Last Updated: May 1, 2026

A Protocol for Real-time 3D Single Particle Tracking
10:16

A Protocol for Real-time 3D Single Particle Tracking

Published on: January 3, 2018

14.5K
Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules
10:20

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Published on: September 5, 2019

7.9K
Mass-Sensitive Particle Tracking to Characterize Membrane-Associated Macromolecule Dynamics
13:30

Mass-Sensitive Particle Tracking to Characterize Membrane-Associated Macromolecule Dynamics

Published on: February 18, 2022

7.7K

Area of Science:

  • Biophysics
  • Cell Biology
  • Microscopy

Background:

  • Protein organization and dynamics are crucial for cellular functions.
  • Single-molecule imaging provides precise data on molecular interactions.
  • Traditional single-particle tracking (SPT) has limitations in statistical power due to low labeling concentrations.

Purpose of the Study:

  • To review the principles of single-particle tracking (SPT) microscopy.
  • To highlight the advantages of high-density SPT for studying biomolecular organization and dynamics.
  • To illustrate recent biological applications of advanced SPT techniques.

Main Methods:

  • Review of single-particle tracking (SPT) principles.
  • Discussion of high-density single-molecule-based super-resolution techniques.
  • Integration of microscopy, image analysis, and labeling strategies.

Main Results:

  • High-density SPT allows monitoring thousands of biomolecules on the minute timescale.
  • This technique provides unprecedented insight into molecular organization and dynamics.
  • Improved statistical power enables more robust analysis of cellular compounds.

Conclusions:

  • Single-particle tracking (SPT) is a powerful interdisciplinary technique for studying molecular interactions.
  • High-density SPT significantly enhances the ability to extract large statistics.
  • Advanced SPT methods offer new avenues for understanding cellular mechanisms at the single-molecule level.