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Related Experiment Video

Updated: May 1, 2026

Temporal Analysis of the Nuclear-to-cytoplasmic Translocation of a Herpes Simplex Virus 1 Protein by Immunofluorescent Confocal Microscopy

Temporal Analysis of the Nuclear-to-cytoplasmic Translocation of a Herpes Simplex Virus 1 Protein by Immunofluorescent Confocal Microscopy

Published on: November 4, 2018

Multifluorescence live analysis of herpes simplex virus type-1 replication.

Michael Seyffert¹, Anna Paula de Oliveira, Cornel Fraefel

¹Institute of Virology, University of Zurich, Winterthurerstr. 266a, 8057, Zurich, Switzerland, mseyffert@vetvir.uzh.ch.

Methods in Molecular Biology (Clifton, N.J.)

|March 28, 2014

Summary

Live cell markers like autofluorescent proteins enable spatial and temporal monitoring of virus replication. This study details multi-fluorescent analysis of herpes simplex virus type-1 using live-cell confocal microscopy.

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Last Updated: May 1, 2026

Temporal Analysis of the Nuclear-to-cytoplasmic Translocation of a Herpes Simplex Virus 1 Protein by Immunofluorescent Confocal Microscopy

Temporal Analysis of the Nuclear-to-cytoplasmic Translocation of a Herpes Simplex Virus 1 Protein by Immunofluorescent Confocal Microscopy

Published on: November 4, 2018

Live Cell Imaging of Alphaherpes Virus Anterograde Transport and Spread

Live Cell Imaging of Alphaherpes Virus Anterograde Transport and Spread

Published on: August 16, 2013

Single-Cell Multiplexed Fluorescence Imaging to Visualize Viral Nucleic Acids and Proteins and Monitor HIV, HTLV, HBV, HCV, Zika Virus, and Influenza Infection

Single-Cell Multiplexed Fluorescence Imaging to Visualize Viral Nucleic Acids and Proteins and Monitor HIV, HTLV, HBV, HCV, Zika Virus, and Influenza Infection

Published on: October 29, 2020

Area of Science:

Virology
Cell Biology
Microscopy

Background:

Live cell markers, such as autofluorescent proteins, are crucial for understanding virus life cycles.
Monitoring viral replication in real-time provides insights into spatial and temporal dynamics.

Purpose of the Study:

To describe the multi-fluorescent analysis of herpes simplex virus type-1 (HSV-1).
To utilize live-cell confocal laser scanning microscopy for studying HSV-1 replication.

Main Methods:

Application of multi-fluorescent labeling techniques.
Live-cell confocal laser scanning microscopy for high-resolution imaging.
Analysis of multi-compartment structures within HSV-1 infected cells.

Main Results:

Demonstration of spatial and temporal monitoring of viral structures.
Visualization of different compartments involved in HSV-1 replication.
Successful application of autofluorescent proteins for live-cell imaging of HSV-1.

Conclusions:

Multi-fluorescent live-cell microscopy is a powerful tool for dissecting complex viral life cycles.
This approach enhances understanding of herpes simplex virus type-1 replication dynamics.
Autofluorescent protein labeling facilitates detailed analysis of viral and cellular interactions.