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Class I MHC-restricted cytotoxic T cells efficiently recognize haemagglutinin that is defective in protein folding
P A Jennings1, D B Boyle, M E Andrew
1CSIRO Division of Biotechnology, Laboratory for Molecular Biology, North Ryde, NSW, Australia.
Cytotoxic T-cell recognition of an engineered variant of the influenza viral haemagglutinin (HA), expressed in vaccinia virus, was investigated. We show that the insertion of a Foot-and-Mouth Disease virus (FMDV) immunogenic peptide into the HA results in major disruption of its higher order structure with intracellular rather than cell surface localization accompanying the loss of conformational epitopes detected by antibody. In contradistinction to antibody, recognition of the chimaeric molecule by HA-specific class I-restricted cytotoxic T-cells was unimpaired, demonstrating that class I-specific T-cells recognize, in majority, continuous epitopes rather than conformational epitopes in the HA.
Cytotoxic T-cell recognition of an engineered variant of the influenza viral haemagglutinin (HA), expressed in vaccinia virus, was investigated. We show that the insertion of a Foot-and-Mouth Disease virus (FMDV) immunogenic peptide into the HA results in major disruption of its higher order structure with intracellular rather than cell surface localization accompanying the loss of conformational epitopes detected by antibody. In contradistinction to antibody, recognition of the chimaeric molecule by HA-specific class I-restricted cytotoxic T-cells was unimpaired, demonstrating that class I-specific T-cells recognize, in majority, continuous epitopes rather than conformational epitopes in the HA.