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Organic solvent tolerant lipases and applications.

Shivika Sharma1, Shamsher S Kanwar1

  • 1Department of Biotechnology, Himachal Pradesh University, Summer Hill, Shimla 171 005, India.

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Lipases are versatile enzymes that function in both aqueous and organic solvents. This review highlights organic solvent-tolerant lipases, detailing their properties and significant industrial applications as biocatalysts.

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Area of Science:

  • Biocatalysis
  • Enzyme Technology
  • Organic Chemistry

Background:

  • Lipases are enzymes capable of catalyzing reactions in both aqueous and organic solvents.
  • Organic solvents enhance substrate solubility and facilitate product recovery in enzymatic reactions.
  • The development of organic solvent-tolerant lipases is crucial for various industrial biotransformations.

Purpose of the Study:

  • To review the properties of organic solvent-tolerant lipases.
  • To explore the industrial applications of lipases in non-aqueous media.
  • To present lipases as key biocatalysts for current and future industrial processes.

Main Methods:

  • Literature review focusing on organic solvent tolerance in lipases.
  • Analysis of lipase activity, stability, and selectivity in organic media.
  • Examination of commercial applications of immobilized lipases in non-aqueous synthesis.

Main Results:

  • Organic solvent-tolerant lipases exhibit enhanced activity, stability, regiospecificity, and stereoselectivity.
  • Organic media improve substrate solubility and shift reaction equilibrium towards synthesis.
  • Immobilized lipases are commercially used for fatty acid ester production in non-aqueous solvents.

Conclusions:

  • Lipases are valuable biocatalysts due to their tolerance and effectiveness in organic solvents.
  • The unique properties of organic solvent-tolerant lipases offer significant advantages for industrial synthesis.
  • Continued research into these enzymes will drive future innovations in biocatalysis.