Allyl isothiocyanate increases MRP1 function and expression in a human bronchial epithelial cell line
Dian-lei Wang1, Chen-yin Wang1, Yin Cao2
1Anhui University of Chinese Medicine, Hefei, Anhui 230038, China.
Oxidative Medicine and Cellular Longevity
|March 28, 2014
Summary
Allyl isothiocyanate (AITC) increases the expression and activity of multidrug resistance-associated protein 1 (MRP1) in human bronchial cells. This suggests AITC may offer therapeutic benefits for lung diseases.
Area of Science:
- Cell Biology
- Pharmacology
- Biochemistry
Background:
- Multidrug resistance-associated protein 1 (MRP1) is an ABC transporter crucial for lung cell protection against toxins.
- Understanding MRP1 regulation is vital for developing treatments for lung diseases.
Purpose of the Study:
- To investigate the effects of allyl isothiocyanate (AITC) on MRP1 expression and activity in human bronchial epithelial cells (16HBE14o-).
Main Methods:
- Real-time PCR and Western blotting were used to analyze MRP1 mRNA and protein levels.
- Functional flow cytometry assessed MRP1 transporter activity using carboxyfluorescein efflux.
Main Results:
- AITC significantly increased MRP1 mRNA and protein expression in a concentration-dependent manner.
- AITC enhanced MRP1-dependent carboxyfluorescein efflux, indicating increased transporter activity.
- Upregulation occurred at AITC concentrations ranging from 1-40 μM.
Conclusions:
- AITC effectively upregulates both the expression and activity of MRP1 in human bronchial epithelial cells.
- The findings suggest AITC holds potential therapeutic value for treating lung diseases by enhancing cellular defense mechanisms.
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