Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Bacteriophages01:26

DNA Bacteriophages

1.6K
Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...
1.6K
Bacterial Phylum Actinobacteria01:30

Bacterial Phylum Actinobacteria

854
Coryneform bacteria are gram-positive, aerobic, nonmotile rods that exhibit irregular, club-shaped, or V-shaped arrangements. Their V-shape results from snapping division, where the inner cell wall layer forms the cross-wall, while the outer layer remains intact until it ruptures on one side, causing the daughter cells to bend away.The primary genera are Corynebacterium and Arthrobacter. Corynebacterium includes diverse species, ranging from saprophytes to pathogens like Corynebacterium...
854
Lysogenic Cycle of Bacteriophages00:43

Lysogenic Cycle of Bacteriophages

49.8K
In contrast to the lytic cycle, phages infecting bacteria via the lysogenic cycle do not immediately kill their host cell. Instead, they combine their genome with the host genome, allowing the bacteria to replicate the phage DNA along with the bacterial genome. The incorporated copy of the phage genome is called the prophage. Some prophages can re-activate and enter the lytic cycle. This often occurs in response to a perturbation, such as DNA damage, but can also transpire in the absence of...
49.8K
Viral Replication: Lysogenic Cycle01:16

Viral Replication: Lysogenic Cycle

2.7K
The lysogenic cycle is a crucial viral replication strategy that allows bacteriophages to persist within host cells without immediately destroying them. This process is primarily observed in temperate phages, such as bacteriophage lambda (λ), which infects Escherichia coli. The cycle allows the viral genome to persist across bacterial generations while keeping host cells viable.Integration of the Viral GenomeUpon infection, bacteriophage lambda attaches to the bacterial surface and injects...
2.7K
Bacterial Phylum Tenericutes01:24

Bacterial Phylum Tenericutes

673
The phylum Tenericutes, which includes the single class Mollicutes, comprises bacteria that lack cell walls. The term "Mollicutes" derives from the Latin word mollis, meaning "soft." These organisms are among the smallest known and are commonly referred to as mycoplasmas due to the prominence of the genus Mycoplasma, which includes well-known human pathogens. Despite their inability to stain gram-positively (a result of their lack of cell walls), mycoplasmas are phylogenetically related to the...
673
Genomic DNA in Prokaryotes00:46

Genomic DNA in Prokaryotes

42.4K
The genome of most prokaryotic organisms consists of double-stranded DNA organized into one circular chromosome in a region of cytoplasm called the nucleoid. The chromosome is tightly wound, or supercoiled, for efficient storage. Prokaryotes also contain other circular pieces of DNA called plasmids. These plasmids are smaller than the chromosome and often carry genes that confer adaptive functions, such as antibiotic resistance.
Genomic Diversity in Bacteria
Although bacterial genomes are much...
42.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mycobacterium marinum MMAR_0267-regulated copper utilization facilitates bacterial escape from phagolysosome.

Communications biology·2024
Same author

Mycobacterium LacI-type Transcription Regulator Rv3575c Affects Host Innate Immunity by Regulating Bacterial <i>mce4</i> Operon-Mediated Cholesterol Transport.

ACS infectious diseases·2024
Same author

Laser-induced stripping defect for highly selective electrochemical quantification of dopamine: Anti-interference from other catecholamine neurotransmitters.

Talanta·2024
Same author

Role of Early On-Treatment Serum HBV RNA Declines in Predicting Hepatocellular Carcinoma Risk in Patients With Chronic Hepatitis B.

Clinical gastroenterology and hepatology : the official clinical practice journal of the American Gastroenterological Association·2024
Same author

Golden insights from a silver superatom.

National science review·2024
Same author

Mycobacterium smegmatis MfpC is a GEF that regulates mfpA translationally to alter the fluoroquinolone efficacy.

Communications biology·2024

Related Experiment Video

Updated: May 1, 2026

Identification of Virulence Markers of Mycobacterium abscessus for Intracellular Replication in Phagocytes
08:34

Identification of Virulence Markers of Mycobacterium abscessus for Intracellular Replication in Phagocytes

Published on: September 27, 2018

8.3K

Prophage-like elements present in Mycobacterium genomes.

Xiangyu Fan, Longxiang Xie, Wu Li

  • 1Institute of Modern Biopharmaceuticals, State Key Laboratory breeding base of Three Gorges Eco-environment and Bioresources, Eco-Environment Key Laboratory of the Three Gorges Reservoir Region, Ministry of Education, School of Life Sciences, Southwest University, 400715 Chongqing, China. georgex@swu.edu.cn.

BMC Genomics
|March 29, 2014
PubMed
Summary
This summary is machine-generated.

This study systematically characterizes mycobacteriophages, revealing thirty-three prophages and prophage-like elements. Eleven novel phages were identified, enhancing our understanding of Mycobacterium biology and phage genomics.

More Related Videos

Analysis of the Lipid Composition of Mycobacteria by Thin Layer Chromatography
07:42

Analysis of the Lipid Composition of Mycobacteria by Thin Layer Chromatography

Published on: April 16, 2021

8.5K
Preparation of Mycobacterium Tuberculosis Culture Filtrate to Understand TB Pathogenesis
07:32

Preparation of Mycobacterium Tuberculosis Culture Filtrate to Understand TB Pathogenesis

Published on: March 28, 2025

1.3K

Related Experiment Videos

Last Updated: May 1, 2026

Identification of Virulence Markers of Mycobacterium abscessus for Intracellular Replication in Phagocytes
08:34

Identification of Virulence Markers of Mycobacterium abscessus for Intracellular Replication in Phagocytes

Published on: September 27, 2018

8.3K
Analysis of the Lipid Composition of Mycobacteria by Thin Layer Chromatography
07:42

Analysis of the Lipid Composition of Mycobacteria by Thin Layer Chromatography

Published on: April 16, 2021

8.5K
Preparation of Mycobacterium Tuberculosis Culture Filtrate to Understand TB Pathogenesis
07:32

Preparation of Mycobacterium Tuberculosis Culture Filtrate to Understand TB Pathogenesis

Published on: March 28, 2025

1.3K

Area of Science:

  • Microbiology
  • Genomics
  • Virology

Background:

  • Prophages are key elements in bacterial genomes, influencing host traits like virulence and evolution.
  • While prophages in some bacteria are well-studied, those in Mycobacterium remain poorly understood.

Purpose of the Study:

  • To systematically identify and characterize prophages within Mycobacterium genomes.
  • To analyze the genomic organization and phylogenetic relationships of these mycobacteriophages.

Main Methods:

  • Comprehensive search of public genome databases (GenBank, WGS) and literature.
  • Detailed analysis of thirty-three identified prophages, including eleven novel ones.
  • Comparative genomics to determine phylogenetic relationships and classifications.

Main Results:

  • Thirty-three prophages and prophage-like elements were identified in Mycobacterium genomes.
  • Eleven of these prophages were newly discovered and analyzed for the first time.
  • Comparative genomics revealed that phi172_2 belongs to Cluster A, and several others form a distinct homology group.

Conclusions:

  • This research provides the first systematic characterization of mycobacteriophages.
  • The findings offer crucial insights into the genomic organization and phylogeny of these elements.
  • This work advances the understanding of Mycobacterium biology and phage-host interactions.