Explanatory chapter: troubleshooting protein expression: what to do when the protein is not soluble

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Recombinant Protein Expression for Structural Biology in HEK 293F Suspension Cells: A Novel and Accessible Approach
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Published on: October 16, 2014

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Krisna C Duong-Ly1, Sandra B Gabelli1

  • 1Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, USA.

Methods in Enzymology
|March 29, 2014
PubMed

Keywords:
Cell lysisDifferential scanning fluorimetry (DSF)IPTGImmunoblottingSDS-PAGESmall ubiquitin-like modifier (SUMO)Troubleshooting protein expression

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