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Related Concept Videos

Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

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The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which...
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Canonical Wnt Signaling Pathway02:54

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Non-Canonical Wnt Signaling Pathways01:41

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Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
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Notch Signaling Pathway03:14

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The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
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Related Experiment Video

Updated: May 1, 2026

Using Confocal Analysis of Xenopus laevis to Investigate Modulators of Wnt and Shh Morphogen Gradients
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Modulating and measuring Wingless signalling.

Jean-Paul Vincent1

  • 1MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London NW71AA, United Kingdom.

Methods (San Diego, Calif.)
|March 29, 2014
PubMed
Summary
This summary is machine-generated.

Researchers developed methods to measure and control Wingless (Wnt) signaling in Drosophila. These tools are crucial for understanding its role in development and regeneration.

Keywords:
DrosophilaReporter geneSignallingWingless

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Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands
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Area of Science:

  • Developmental Biology
  • Cell Signaling
  • Genetics

Background:

  • The Wingless (Wnt) signaling pathway is crucial for cellular activities in development, regeneration, and nervous system function.
  • Understanding and manipulating Wnt signaling is essential for biological research.

Purpose of the Study:

  • To describe experimental methods for measuring and modulating Wingless signaling in Drosophila.
  • To highlight the challenges and advancements in detecting Wnt signaling in cell culture and intact tissues.

Main Methods:

  • Development of luciferase-based reporters with TCF-binding sites or target gene fragments.
  • Activation of signaling via Wingless conditioned medium, Shaggy/GSK3 inhibition, or activated Armadillo transfection.
  • Utilizing gene traps in frizzled3 and notum/wingful as endogenous reporters for intact tissues.

Main Results:

  • Established methods for measuring Wingless signaling in Drosophila cell culture using reporter assays.
  • Identified challenges in real-time, sensitive detection of Wnt signaling in intact tissues.
  • Demonstrated the utility of endogenous gene traps as alternative reporters.

Conclusions:

  • Various tools are available for measuring and modulating Wingless signaling in Drosophila.
  • Further improvements in reporter systems are needed for sensitive detection in intact tissues.
  • Genetic tools, including RNAi and genome engineering, offer precise manipulation of the Wnt pathway.