Related Experiment Video

Updated: May 1, 2026

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
08:33

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Published on: December 5, 2017

13.7K

Depletion of pre-mRNA splicing factor Cdc5L inhibits mitotic progression and triggers mitotic catastrophe

R Mu1, Y-B Wang1, M Wu1

  • 1State Key Laboratory of Proteomics, Institute of Basic Medical Sciences, National Center of Biomedical Analysis, Beijing, China.

Cell Death & Disease
|March 29, 2014
PubMed

Insights

Cell division cycle 5-like (Cdc5L), a splicing factor, regulates mitosis. Depleting Cdc5L causes mitotic arrest and DNA damage, suggesting its potential as an anti-cancer drug target.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Cancer Research

Background:

  • Targeting mitotic progression is a key anti-cancer strategy.
  • Identifying novel molecular targets in mitosis is crucial for drug development.

Purpose of the Study:

  • To investigate the role of cell division cycle 5-like (Cdc5L) in mitotic progression.
  • To explore Cdc5L as a potential therapeutic target for cancer.

Main Methods:

  • Depletion of Cdc5L using knockdown techniques.
  • Analysis of mitotic progression, chromosome alignment, and spindle assembly checkpoint.
  • Assessment of kinetochore-microtubule attachment and DNA damage.
  • Genome-wide gene expression analysis.
  • Investigation of pre-mRNA splicing efficiency.

Main Results:

  • Cdc5L depletion induced mitotic arrest, chromosome misalignment, and sustained spindle assembly checkpoint activation.
  • Defects were linked to impaired kinetochore-microtubule attachment and DNA damage.
  • Cdc5L knockdown affected the expression and splicing of mitosis and DNA damage response genes.
  • Cdc5L is highly expressed in cervical tumors and osteosarcoma.
  • Downregulation of Cdc5L reduced tumor cell viability.

Conclusions:

  • Cdc5L is a critical regulator of mitotic progression.
  • Cdc5L plays a role in maintaining genomic stability by influencing gene expression and splicing.
  • Cdc5L represents a promising therapeutic target for cervical and osteosarcoma treatment.

More Related Videos

Studying Proteolysis of Cyclin B at the Single Cell Level in Whole Cell Populations10:54

Studying Proteolysis of Cyclin B at the Single Cell Level in Whole Cell Populations

Published on: September 17, 2012

10.3K
Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay12:26

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Published on: May 3, 2018

18.3K

Related Concept Videos

Separation of Sister Chromatids02:17

Separation of Sister Chromatids

At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
3.6K
Anaphase Promoting Complex00:50

Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
2.5K
The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
2.9K
Nonsense-mediated mRNA Decay02:27

Nonsense-mediated mRNA Decay

The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
9.4K
Nonsense-mediated mRNA Decay02:27

Nonsense-mediated mRNA Decay

2.3K
M-Cdk Drives Transition Into Mitosis02:15

M-Cdk Drives Transition Into Mitosis

Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
5.3K