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An activity stain for proteins containing noncovalently bound flavin adenine dinucleotide
1Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710.
Analytical Biochemistry
|November 1, 1988
Abstract:
A procedure has been developed to allow the visualization of FAD-containing proteins on polyacrylamide gels. The technique is based on the reconstitution of apo-D-amino acid oxidase with FAD and is thus specific for this cofactor. The stain is sensitive enough to detect 5 pmol of FAD and is therefore useful for the detection of flavoproteins in systems as complex as crude tissue or bacterial extracts.