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A method for RNA isolation from marine macro-algae.

X Su1, A Gibor

  • 1Department of Biological Sciences, University of California, Santa Barbara 93106.

Analytical Biochemistry
|November 1, 1988
PubMed
Summary
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Marine algae contain compounds that hinder RNA isolation and activity. This study developed methods using differential precipitation and reducing agents to successfully isolate active RNA from brown, red, and green algae.

Area of Science:

  • Marine Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Marine macro-algae contain sulfated, carboxylic polysaccharides, and polyphenols.
  • These compounds interfere with RNA isolation and inhibit RNA activity in vitro.

Purpose of the Study:

  • To develop effective methods for isolating intact and active RNA from marine macro-algae.
  • To overcome the inhibitory effects of algal polysaccharides and polyphenols on RNA.

Main Methods:

  • Differential precipitation of RNA and carbohydrates in high salt concentrations to remove acidic carbohydrates.
  • Utilizing strong reducing agents to prevent the oxidation of polyphenols and protect RNA from inactivation.

Main Results:

  • Successful isolation of RNA from three species of marine algae: brown alga (Macrocystis pyrifera), red alga (Porphyra schizophylla), and green alga (Enteromorpha intestinalis).

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  • Isolated messenger RNA (mRNA) from the total RNA was confirmed to be translationally active, indicating its suitability for downstream applications.

    • Conclusions:

    • The developed methods effectively remove inhibitory compounds from marine algae.
    • High-quality, translationally active RNA can be obtained from diverse marine macro-algae species, enabling further molecular studies.