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The Fanconi anemia group C protein interacts with uncoordinated 5A and delays apoptosis

FengFei Huang1, Manel Ben Aissa1, Audrey Magron1

  • 1Department of Pediatrics, Centre Hospitalier Universitaire de Québec, Québec, Québec, Canada.

Plos One
|March 29, 2014
PubMed

Insights

Fanconi anemia group C protein (FANCC) interacts with the pro-apoptotic receptor UNC5A. FANCC suppresses UNC5A-induced apoptosis, suggesting a role in development.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genetics

Background:

  • Fanconi anemia group C protein (FANCC) is crucial for genomic stability within the Fanconi anemia (FA) network.
  • FANCC regulates apoptosis via caspase-mediated processing, primarily functioning in the cytoplasm.

Purpose of the Study:

  • To investigate the function of FANCC proteolytic fragments by identifying their binding partners.
  • To elucidate the interaction between FANCC and its cleavage products in the context of apoptosis regulation.

Main Methods:

  • Yeast two-hybrid screening was employed using caspase-mediated FANCC cleavage products.
  • Co-immunoprecipitation and apoptosis assays were utilized to validate interactions and functional consequences.

Main Results:

  • The dependence receptor uncoordinated-5A (UNC5A) was identified as a binding partner for FANCC fragments.
  • FANCC physically interacts with the intracellular death domain of UNC5A.
  • FANCC presence reduces UNC5A-mediated apoptosis, while FANCC depletion enhances it.

Conclusions:

  • FANCC interferes with the pro-apoptotic functions of UNC5A.
  • The interaction between FANCC and UNC5A suggests a novel role for FANCC in modulating apoptosis and potentially in developmental processes.

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