Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Synthetic circuits for cell ratio control.

Nature·2026
Same author

Design principles for adaptive and evolving engineered living materials.

Current opinion in biotechnology·2025
Same author

Deep generative models design mRNA sequences with enhanced translational capacity and stability.

Science (New York, N.Y.)·2025
Same author

Mitigation of Doxorubicin Cardiotoxicity With Synergistic miRNA Combinations Identified Using Combinatorial Genetics en masse (CombiGEM).

JACC. CardioOncology·2025
Same author

Rapid Universal Detection of High-Risk and Low-Abundance Microbial Contaminations in CAR-T Cell Therapy.

Small methods·2025
Same author

Sleeping Beauty mRNA-LNP enables stable rAAV transgene expression in mouse and NHP hepatocytes and improves vector potency.

Molecular therapy : the journal of the American Society of Gene Therapy·2024

Related Experiment Video

Updated: Dec 21, 2025

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
10:46

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Published on: October 18, 2022

2.2K

Digital and analog gene circuits for biotechnology.

Nathaniel Roquet1, Timothy K Lu

  • 1Synthetic Biology Group, Research Lab of Electronics, Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, MA, USA; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA; Harvard Biophysics Program, Boston, MA, USA.

Biotechnology Journal
|March 29, 2014
PubMed
Summary

Synthetic gene circuits offer dynamic control for microbial biotechnology, overcoming limitations of static engineering. These adaptable systems promise more efficient and versatile biochemical production in industrial applications.

Keywords:
Analog gene circuitDigital gene circuitMemoryMetabolic engineeringSynthetic biology

More Related Videos

A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression
11:23

A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression

Published on: October 6, 2019

10.6K
Automated Robotic Liquid Handling Assembly of Modular DNA Devices
11:22

Automated Robotic Liquid Handling Assembly of Modular DNA Devices

Published on: December 1, 2017

12.7K

Related Experiment Videos

Last Updated: Dec 21, 2025

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
10:46

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Published on: October 18, 2022

2.2K
A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression
11:23

A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression

Published on: October 6, 2019

10.6K
Automated Robotic Liquid Handling Assembly of Modular DNA Devices
11:22

Automated Robotic Liquid Handling Assembly of Modular DNA Devices

Published on: December 1, 2017

12.7K

Area of Science:

  • Biotechnology
  • Synthetic Biology
  • Metabolic Engineering

Background:

  • Static metabolic engineering strategies have advanced industrial biotechnology but face inefficiencies during large-scale microbial culture due to variable conditions.
  • Dynamic synthetic gene circuits can address these inefficiencies by enabling microbial cells to sense and adapt to changing environments.

Purpose of the Study:

  • To review the design and application of digital and analog synthetic gene circuits in biotechnology.
  • To explore the potential benefits of interconversion, memory, and multi-signal integration within these dynamic circuits.

Main Methods:

  • Review of existing literature on synthetic gene circuit design and application.
  • Categorization of circuits into digital and analog classes.
  • Discussion of advanced circuit functionalities like interconversion, memory, and multi-signal integration.

Main Results:

  • Synthetic gene circuits can be broadly classified into digital and analog types.
  • These circuits enable dynamic regulation of cellular processes, enhancing adaptability.
  • Advanced features like digital-analog interconversion, memory, and multi-signal integration offer further control and programmability.

Conclusions:

  • Synthetic gene circuits represent a powerful tool for enhancing the efficiency and scope of biochemical production using microbial systems.
  • Dynamic regulation through gene circuits can overcome the scalability challenges associated with static engineering approaches.
  • Future applications of synthetic gene circuits in biotechnology hold significant promise for next-generation bioprocessing and cellular computation.