Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Quantitative studies of keratin expression with the post-embedding immunogold labeling method.

M Haftek1, M C Chignol, J Thivolet

  • 1CNRS UA 601, INSERM U 209, Clinique Dermatologique, Hôpital E. Herriot, Lyon, France.

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
|May 1, 1989
PubMed
Summary

Acidic keratin (type I) 56.5 KD is found in suprabasal keratinocytes of human epidermis. Its immunoreactivity peaks in the spinous layer and decreases in the granular and horny layers.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Brief report: successive occurrence of T-cell and B-cell lymphomas after renal transplantation in a patient with multiple cutaneous squamous-cell carcinomas.

The New England journal of medicine·1992
Same author

Expression of neuron-specific enolase immunoreactivity by cutaneous and extracutaneous Langerhans-cell histiocytoses ("X").

The Journal of dermatology·1992
Same author

Influence of fixation on human papillomavirus DNA detection in frozen and embedded paraffin lesions by in situ hybridization with biotinylated probes.

Pathology, research and practice·1992
Same author

In vitro HIV-1 entry and replication in Langerhans cells may clarify the HIV-1 genome detection by PCR in epidermis of seropositive patients.

The Journal of investigative dermatology·1992
Same author

Alpha-6 (CD 49f) integrin expression in genetic and acquired bullous skin diseases. A comparison of its distribution with bullous pemphigoid antigen.

Journal of cutaneous pathology·1992
Same author

Anti-CD4 monoclonal antibody therapy in severe psoriasis.

Journal of autoimmunity·1992

Area of Science:

  • Dermatology
  • Cell Biology
  • Biochemistry

Background:

  • Keratins are intermediate filament proteins crucial for epidermal structure and function.
  • Acidic (type I) keratins, like 56.5 KD, play specific roles in keratinocyte differentiation.
  • Understanding keratin distribution is key to comprehending epidermal development and pathology.

Purpose of the Study:

  • To ultrastructurally localize and quantify the 56.5 KD acidic keratin in normal human epidermis.
  • To investigate the expression pattern of 56.5 KD keratin during keratinocyte terminal differentiation.
  • To validate the specificity of the monoclonal antibody KL1 for 56.5 KD keratin.

Main Methods:

  • Post-embedding immunogold labeling technique was employed.
  • The monoclonal antibody KL1 was used to detect 56.5 KD keratin.

Related Experiment Videos

  • Quantitative analysis of immunogold labeling was performed across epidermal layers.

    • Main Results:

    • 56.5 KD keratin was localized in keratin intermediate filament bundles of suprabasal keratinocytes.
    • Immunoreactivity increased from the first suprabasal layer to a maximum in the outmost spinous layer.
    • A significant decrease in labeling was observed in the granular and horny layers, possibly due to degradation or masking.

    Conclusions:

    • The 56.5 KD keratin is specifically expressed in terminally differentiating suprabasal keratinocytes.
    • The study highlights the dynamic changes in keratin expression during epidermal differentiation.
    • Appropriate control studies are essential for validating post-embedding immunogold labeling methods.