Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

An Integrative Network Analysis Framework for Identifying Altered Glycosylation Pathways Associated with Autism Spectrum Disorder.

Genes·2026
Same author

Differential alpha-gal expression during <i>Amblyomma hebraeum</i> and <i>Rhipicephalus evertsi</i> tick feeding and development: A driver for the development of alpha-gal syndrome in South Africa.

The journal of allergy and clinical immunology. Global·2026
Same author

Effects of race and ethnicity on hematopoietic stem cell transplant outcomes in acute myeloid leukemia: a systematic review and meta-analysis.

Frontiers in oncology·2025
Same author

Development of an in vitro fibrotic scar model of spinal cord injury using macromolecular crowding.

Journal of neuroscience methods·2025
Same author

Immobilized β-galactosidase BgaC from Bifidobacterium adolescentis retains stability and activity during repeated cycles of use.

Applied microbiology and biotechnology·2025
Same author

The effect of macromolecular crowders on deposition of extracellular matrix in astrocyte cultures.

Cell and tissue research·2025

Related Experiment Video

Updated: May 1, 2026

Subtyping of Campylobacter jejuni ssp. doylei Isolates Using Mass Spectrometry-based PhyloProteomics MSPP
09:43

Subtyping of Campylobacter jejuni ssp. doylei Isolates Using Mass Spectrometry-based PhyloProteomics MSPP

Published on: October 30, 2016

8.1K

Campylobacter jejuni strain discrimination and temperature-dependent glycome expression profiling by lectin

Michelle Kilcoyne1, Marcus E Twomey2, Jared Q Gerlach1

  • 1Glycoscience Group, National Centre for Biomedical Engineering Science, National University of Ireland, Galway, Ireland.

Carbohydrate Research
|April 1, 2014
PubMed
Summary

Campylobacter jejuni surface carbohydrates change with temperature, impacting its adaptation between human and avian hosts. Understanding these glycan changes offers insights into bacterial gastroenteritis and Guillain-Barré syndrome triggers.

Keywords:
Campylobacter jejuniCapsular polysaccharideLectin microarrayLipooligosaccharideLipopolysaccharideVirulence factors

More Related Videos

Author Spotlight: MAPP Protocol &#8211; Advancing Glycan Analysis
07:12

Author Spotlight: MAPP Protocol – Advancing Glycan Analysis

Published on: September 29, 2023

2.6K
Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis
12:02

Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis

Published on: April 11, 2016

14.2K

Related Experiment Videos

Last Updated: May 1, 2026

Subtyping of Campylobacter jejuni ssp. doylei Isolates Using Mass Spectrometry-based PhyloProteomics MSPP
09:43

Subtyping of Campylobacter jejuni ssp. doylei Isolates Using Mass Spectrometry-based PhyloProteomics MSPP

Published on: October 30, 2016

8.1K
Author Spotlight: MAPP Protocol &#8211; Advancing Glycan Analysis
07:12

Author Spotlight: MAPP Protocol – Advancing Glycan Analysis

Published on: September 29, 2023

2.6K
Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis
12:02

Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis

Published on: April 11, 2016

14.2K

Area of Science:

  • Microbiology
  • Glycobiology
  • Infectious Diseases

Background:

  • Campylobacter jejuni is a major cause of bacterial gastroenteritis and Guillain-Barré syndrome.
  • C. jejuni transitions between pathogenic (human) and commensal (avian) lifestyles.
  • Host temperature is a potential stimulus for C. jejuni adaptation.

Purpose of the Study:

  • To investigate the modulation of C. jejuni's surface glycome in response to temperature.
  • To elucidate temperature-dependent mechanisms of C. jejuni colonization and pathogenesis.

Main Methods:

  • Culturing C. jejuni strains (81116 and 81-176) at human (37°C) and avian (42°C) host temperatures.
  • Profiling whole cells using lectin microarrays with diverse specificities.
  • Confirming lipooligosaccharide (LOS) changes via SDS-PAGE.

Main Results:

  • Distinct glycan profiles were observed for both strains at different temperatures.
  • Strain 81116 showed reduced LPS-like structure dominance at 42°C.
  • Strain 81-176 exhibited altered capsular polysaccharide (CPS) and LOS distribution at 42°C, confirmed by SDS-PAGE.

Conclusions:

  • Temperature significantly modulates the C. jejuni surface glycome.
  • Differential glycosylation is crucial for C. jejuni host-specific tropism.
  • Lectin microarrays provide valuable insights into bacterial adaptation mechanisms.