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Sexual Transmission of American Trypanosomes from Males and Females to Naive Mates
Published on: January 27, 2019
ORC1/CDC6 and MCM7 distinct associate with chromatin through Trypanosoma cruzi life cycle
Simone Calderano1, Patricia Godoy2, Daiane Soares1
1Laboratório Especial de Ciclo Celular, Instituto Butantan, São Paulo, Brazil; Center of Toxins, Immune Response and Cell Signaling - CeTICS, Brazil.
Abstract:
Trypanosoma cruzi alternates between replicative and non-replicative stages. We analyzed the expression of components of the pre-replication machinery TcORC1/CDC6 and TcMCM7 and their interaction with DNA in all T. cruzi stages. TcORC1/CDC6 remains in the nuclear space during all stages of the life cycle and interacts with DNA in the replicative stages; however, it does not bind to DNA in the non-replicative forms. Moreover, TcMCM7 is not present in the non-replicative stages. These data suggest that the lacking of DNA replication during the T. cruzi life cycle may be a consequence of the blocking of TcORC1/CDC6-DNA interaction and of the down regulation of the TcMCM7 expression.
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