Related Experiment Video

Updated: May 1, 2026

miRNA Expression Analyses in Prostate Cancer Clinical Tissues
11:29

miRNA Expression Analyses in Prostate Cancer Clinical Tissues

Published on: September 8, 2015

10.3K

Mir-23b and miR-130b expression is downregulated in pituitary adenomas

Vincenza Leone1, Concetta Langella1, Daniela D'Angelo1

  • 1Istituto di Endocrinologia ed Oncologia Sperimentale del CNR, c/o Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Scuola di Medicina e Chirurgia di Napoli, Università degli Studi di Napoli "Federico II", Naples, Italy.

Molecular and Cellular Endocrinology
|April 1, 2014
PubMed

Insights

Reduced microRNAs (miRNAs), specifically miR-23b and miR-130b, are linked to pituitary adenomas. Their downregulation may promote tumor growth by increasing target genes like HMGA2 and CCNA2.

Area of Science:

  • Endocrinology
  • Molecular Biology
  • Oncology

Background:

  • MicroRNA (miRNA) deregulation is implicated in tumorigenesis.
  • Thyrotropin induces miR-23b and miR-130b in thyroid cells via cAMP.
  • The role of these miRNAs in pituitary tumorigenesis requires investigation.

Purpose of the Study:

  • To investigate the role of miR-23b and miR-130b in pituitary adenomas (PAs).
  • To analyze the expression levels of miR-23b and miR-130b in various PAs.
  • To determine the functional impact of miR-23b and miR-130b on pituitary cell proliferation and their target genes.

Main Methods:

  • Analysis of miR-23b and miR-130b expression in human pituitary adenomas (GH and NFPA) and normal pituitary tissue.
  • Overexpression studies to assess the effect of miR-23b and miR-130b on cell proliferation and cell cycle progression.
  • Identification of target genes for miR-23b and miR-130b using molecular techniques.

Main Results:

  • miR-23b and miR-130b expression are significantly reduced in GH, gonadotroph, and NFPA adenomas compared to normal pituitary tissue.
  • Overexpression of miR-23b inhibits cell proliferation by arresting cells in G1 phase.
  • Overexpression of miR-130b inhibits cell proliferation by arresting cells in G2 phase.
  • miR-23b targets HMGA2, and miR-130b targets cyclin A2 (CCNA2).
  • Downregulation of these miRNAs correlates with increased levels of their target genes in PAs.

Conclusions:

  • Downregulation of miR-23b and miR-130b is a common feature in pituitary adenomas.
  • These miRNAs act as tumor suppressors by inhibiting cell proliferation.
  • The reduced expression of miR-23b and miR-130b, leading to increased target gene levels, may contribute to pituitary tumorigenesis.
Keywords:
CCNA2HMGA2Pituitary adenomamicroRNA

More Related Videos

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells09:45

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells

Published on: May 21, 2019

8.1K
MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR qPCR08:30

MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR qPCR

Published on: May 16, 2012

24.0K

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
21.1K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K
Receptor Downregulation in MVBs01:15

Receptor Downregulation in MVBs

Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that  lead to cell proliferation, migration, and differentiation. Overexpression of EGFR  stimulates cells to proliferate. Excessive  EGFR...
2.0K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.0K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
3.6K
Hormones of the Pituitary Gland01:27

Hormones of the Pituitary Gland

The small, pea-sized pituitary gland is located at the base of the brain. It is crucial in regulating various bodily functions, from growth to reproduction. The gland is divided into the anterior lobe and the posterior lobe. The secretory cell clusters in the pars distalis of the anterior pituitary lobe are controlled by hypothalamic regulators and synthesize six primary hormones.
The most abundantly secreted hormone from the anterior lobe is the growth hormone, which controls overall growth by...
14.2K