Voltage-driven reversible insertion into and leaving from a lipid bilayer: tuning transmembrane transport of
Wen Si1, Zhan-Ting Li, Jun-Li Hou
1Department of Chemistry, Fudan University, 220 Handan Road, Shanghai 200433 (China).
Abstract:
Three new artificial transmembrane channel molecules have been designed and synthesized by attaching positively charged Arg-incorporated tripeptide chains to pillar[5]arene. Fluorescent and patch-clamp experiments revealed that voltage can drive the molecules to insert into and leave from a lipid bilayer and thus switch on and off the transport of K(+) ions. One of the molecules was found to display antimicrobial activity toward Bacillus subtilis with half maximal inhibitory concentration (IC50 ) of 10 μM which is comparable to that of natural channel-forming peptide alamethicin.
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