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Updated: May 1, 2026
Intravitreal Injection and Quantitation of Infection Parameters in a Mouse Model of Bacterial Endophthalmitis
Published on: February 6, 2021
Lens concentration of ofloxacin and lomefloxacin in an experimental endophthalmitis model
Ramazan Yağci1, Yusuf Oflu1, Server Yağci2
1Ankara Training and Research Hospital, Department of Ophthalmology, Ankara, Turkey.
Unlabelled:
Abstract.
Background:
Bacterial endophthalmitis is a serious complication of ocular surgery and penetrating trauma. The primary causative organisms are strains of Staphylococcus aureus and Staphylococcus epidermidis. Fluoroquinolones are widely used to treat endophthalmitis. There are a few studies on the penetration of fluoroquinolones into the lens in inflamed eyes. A literature search did not identify any data regarding penetration of topical ofloxacin into the lens in normal and inflamed eyes.
Objective:
The aim of this study was to determine the penetration of topical ofloxacin and lomefloxacin into the lens in a rabbit endophthalmitis model.
Methods:
New Zealand white rabbits were randomly divided into 2 groups. The left eyes were infected with an intravitreal inoculation of S aureus. The right eyes were used as a noninoculated control. Groups 1 and 2 received topical ofloxacin and lomefloxacin treatment, respectively, 24 hours after the inoculation. Two drops of the study drugs were instilled in the eyes every 30 minutes for 3 hours and then every 60 minutes for 3 hours. Lens samples were obtained 30 minutes after the last ofloxacin or lomefloxacin drops were administered. High-performance liquid chromatography was used to determine the fluoroquinolone concentration.
Results:
Ten rabbits were equally divided into the 2 treatment groups. There was no significant difference in mean (SD) lens concentrations between the control and inoculated eyes in either treatment group-ofloxacin (0.26 [0.32] μg/mL vs 0.11 [0.05] μg/mL, respectively) and lomefloxacin (0.50 [0.87] μg/mL vs 0.12 [0.08] μg/mL, respectively).
Conclusion:
The results of this small experimental study found that topical ofloxacin and lomefloxacin can accumulate in the crystalline lens after installation. Inflammation did not affect the penetration of ofloxacin or lomefloxacin into the lens.
