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Updated: May 1, 2026

Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration

Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration

Published on: June 16, 2015

Engineering a microvascular capillary bed in a tissue-like collagen construct.

Tijna Alekseeva¹, Ronald E Unger, Christoph Brochhausen

¹1 REPAIR Lab, Institute of Pathology, University Medical Center, Johannes Gutenberg University , Mainz, Germany .

Tissue Engineering. Part A

|April 2, 2014

Summary

This summary is machine-generated.

Plastic compression of collagen gels enables the creation of tissue-like structures. Co-culturing endothelial cells with fibroblasts or osteoblasts in these constructs promotes vessel formation without gel contraction, valuable for tissue engineering.

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Last Updated: May 1, 2026

Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration

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Published on: June 16, 2015

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Area of Science:

Biomaterials Science
Tissue Engineering
Cell Biology

Background:

Plastic compression (PC) of collagen gels is a method for fabricating in vitro constructs that mimic in vivo tissue structures.
Microvascular endothelial cells are crucial for blood vessel formation.

Purpose of the Study:

To investigate the attachment, survival, growth, and endothelial characteristics of microvascular endothelial cells in PC collagen constructs.
To assess the potential of PC collagen constructs for modeling proangiogenic responses and applications in tissue engineering.

Main Methods:

Fabrication of matrix- and cell-rich constructs using plastic compression of collagen gels.
Seeding of microvascular endothelial cells alone or in co-culture with human foreskin fibroblasts (HFF) or human osteoblasts (HOS).
Culturing constructs for 3 weeks and observing cell morphology, survival, and vessel-like structure formation.

Main Results:

Endothelial cells in co-culture with HFF or HOS formed vessel-like structures within 3 weeks without added growth factors.
Endothelial cells cultured alone on PC scaffolds showed typical cobblestone morphology for 21 days.
Absence of gel contraction was observed in co-cultures, even after 3 weeks of fibroblast culture.

Conclusions:

Co-culturing primary endothelial cells with PC collagen constructs containing stromal cells is a valuable method for in vitro modeling of proangiogenic responses.
The absence of gel contraction in these constructs holds significant potential for tissue engineering applications, particularly for skin, to prevent disabling contractures.