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Volatilization

Volatilization

Volatilization gravimetry is an analytical technique that measures the mass lost due to the volatilization of the substance. This technique is used to estimate the amount of volatile material in a sample. To perform this method, heat a known amount of the sample to a high temperature in a crucible or other suitable vessel. The volatile substance in the sample evaporates, and the vapor is completely expelled from the crucible either by heating the sample or bubbling a stream of inert gas through...

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Related Experiment Video

Updated: May 1, 2026

Rapid Collection of Floral Fragrance Volatiles using a Headspace Volatile Collection Technique for GC-MS Thermal Desorption Sampling

Rapid Collection of Floral Fragrance Volatiles using a Headspace Volatile Collection Technique for GC-MS Thermal Desorption Sampling

Published on: December 10, 2019

A robust, simple, high-throughput technique for time-resolved plant volatile analysis in field experiments.

Mario Kallenbach¹, Youngjoo Oh, Elisabeth J Eilers

¹Department of Molecular Ecology, Max Planck Institute for Chemical Ecology, Hans Knöll Straße 8, 07745, Jena, Germany.

The Plant Journal : for Cell and Molecular Biology

|April 2, 2014

Summary

Researchers developed a cost-effective method using silicone tubing (STs) for sampling plant volatiles (PVs) in natural environments. This technique offers reproducible, sensitive, and spatially resolved data, overcoming limitations of artificial settings.

Keywords:

Manduca sexta Nicotiana attenuata headspace analysis indirect defense polydimethylsiloxane silicone tubing technical advance

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Last Updated: May 1, 2026

Rapid Collection of Floral Fragrance Volatiles using a Headspace Volatile Collection Technique for GC-MS Thermal Desorption Sampling

Rapid Collection of Floral Fragrance Volatiles using a Headspace Volatile Collection Technique for GC-MS Thermal Desorption Sampling

Published on: December 10, 2019

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Published on: October 2, 2016

Fruit Volatile Analysis Using an Electronic Nose

Fruit Volatile Analysis Using an Electronic Nose

Published on: March 30, 2012

Area of Science:

Ecology
Plant Science
Analytical Chemistry

Background:

Plant volatiles (PVs) mediate crucial interactions and stress responses.
Current PV sampling often occurs in artificial lab settings, limiting ecological relevance.
Existing methods for natural sampling are costly and technically challenging.

Purpose of the Study:

To introduce a low-cost, efficient method for sampling plant volatiles in natural environments.
To enable high-resolution, reproducible PV measurements under field conditions.
To overcome limitations of traditional laboratory-based and expensive field sampling techniques.

Main Methods:

Utilized polydimethylsiloxane (PDMS) in the form of silicone tubing (STs) for PV headspace sampling.
Employed short pieces of STs (millimeters to centimeters) for minimal disturbance.
Integrated STs with thermal desorption coupled to Gas Chromatography-Mass Spectrometry (GC-MS).

Main Results:

Silicone tubing (STs) proved to be a cost-effective alternative to standard sorbent devices.
STs demonstrated fast absorption kinetics and sufficient capacity for sampling over minutes to hours.
The method yielded reproducible, sensitive, and spatio-temporally resolved quantitative data from natural environments.

Conclusions:

Silicone tubing (STs) offer a practical and affordable solution for in-situ plant volatile sampling.
This technique facilitates biologically meaningful 'snapshots' of PV blends under natural conditions.
The STs-GC-MS approach enhances ecological studies by providing high-quality field data.