Related Experiment Video
Updated: May 1, 2026
Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis
Published on: April 11, 2016
Structural investigation and immunological activity of a heteropolysaccharide from Sargassum fusiforme
Pei Hu1, Rui Xue2, Zhixiong Li2
1Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 501 Haike Rd., Zhangjiang, Pudong, Shanghai 201203, PR China; Department of Clinical Pharmacology, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, 528 Zhangheng Rd., Zhangjiang, Pudong, Shanghai 201203, PR China.
A heteropolysaccharide was isolated from the brown alga, Sargassum fusiforme. The heteropolysaccharide was estimated to have a molecular weight of 11kDa and showed [α]D(20) -62.2420 (c 0.05, H2O). SFPS65-B comprised galactose, glucose, mannose, fucose, and galacturonic acid at a ratio of 3.04:1:1.15:2.82:6.51. Its structural features were investigated using composition analysis, methylation analysis, IR, NMR spectroscopy, and ESI-Q-TOF MS spectroscopy. Results showed that SFPS65-B contained the backbone of →4)-α-GalAp-(1→4)-α-Hexp-(1→4)-α-GalAp-(1→4)-α-Fucp-(1→4)-α-GalAp-(1→. The sulfated unit and terminal fucose residues were attached onto the backbone through the O-2 of some galactose residues. Results also showed that SFPS65-B had a good effect on thymus and spleen indices at a dose of 100mg/kg upon immunosuppression in cyclophosphamide-treated mice.
A heteropolysaccharide was isolated from the brown alga, Sargassum fusiforme. The heteropolysaccharide was estimated to have a molecular weight of 11kDa and showed [α]D(20) -62.2420 (c 0.05, H2O). SFPS65-B comprised galactose, glucose, mannose, fucose, and galacturonic acid at a ratio of 3.04:1:1.15:2.82:6.51. Its structural features were investigated using composition analysis, methylation analysis, IR, NMR spectroscopy, and ESI-Q-TOF MS spectroscopy. Results showed that SFPS65-B contained the backbone of →4)-α-GalAp-(1→4)-α-Hexp-(1→4)-α-GalAp-(1→4)-α-Fucp-(1→4)-α-GalAp-(1→. The sulfated unit and terminal fucose residues were attached onto the backbone through the O-2 of some galactose residues. Results also showed that SFPS65-B had a good effect on thymus and spleen indices at a dose of 100mg/kg upon immunosuppression in cyclophosphamide-treated mice.
