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Related Concept Videos

Cytoplasm01:24

Cytoplasm

6.8K
The cytoplasm consists of organelles and a framework of protein scaffolds called the cytoskeleton suspended in an aqueous solution, the cytosol. The cytosol is a rich broth of water, ions, salts, and various organic molecules.
Protein Folding and Misfolding
The cytoplasm is the location for several cellular processes, including protein synthesis and folding. The aqueous nature of the cytosol promotes protein folding such that the hydrophobic amino acid side chains are buried in the protein...
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Cytoplasm01:16

Cytoplasm

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The cytoplasm consists of organelles and a framework of protein scaffolds called the cytoskeleton suspended in an aqueous solution, the cytosol. The cytosol is a rich broth of water, ions, salts, and various organic molecules.
Protein Folding and Misfolding
The cytoplasm is the location for several cellular processes, including protein synthesis and folding. The aqueous nature of the cytosol promotes protein folding such that the hydrophobic amino acid side chains are buried in the protein...
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Introduction to the Cytoskeleton01:33

Introduction to the Cytoskeleton

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Overview of the Cytoskeleton
The cytoskeleton is a network of protein filaments present within the cell, having three distinct filaments ̶   microfilaments, microtubules, and intermediate filaments. Each has characteristic features that distinguish them, including the dynamics of their assembly and disassembly, mechanical properties, polarity, and the type of molecular motors associated with them. Earlier, they were thought to be present only in eukaryotic cells; however, their...
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Adaptability of Cytoskeletal Filaments01:12

Adaptability of Cytoskeletal Filaments

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The cytoskeleton is a complex dynamic structure performing varied functions based on cellular requirements. The adaptability of the individual filaments in the cytoskeleton determines their ability to perform various functions within the cell. It can undergo rapid reorganization during processes like cell division or remain stable for several hours as in the interphase. The adaptability of these filaments depends on stringent regulatory mechanisms. The microfilament and microtubules of the...
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Cytoskeletal Coordination in Cell Migration01:32

Cytoskeletal Coordination in Cell Migration

4.9K
A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker...
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Cystic Fibrosis: Pathogenesis01:23

Cystic Fibrosis: Pathogenesis

1.1K
Cystic fibrosis (CF), an autosomal recessive disorder, significantly affects the function of exocrine glands. This genetically inherited disease is characterized by the production of thick and sticky mucus, which can severely affect various organs and systems in the body.
CF is primarily caused by a genetic mutation in a chromosome 7 gene coding for the cystic fibrosis transmembrane conductance regulator (CFTR) protein. The most common gene mutation leading to CF is the ΔF508 mutation,...
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Related Experiment Video

Updated: May 1, 2026

In Vitro Analysis of PDZ-dependent CFTR Macromolecular Signaling Complexes
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In Vitro Analysis of PDZ-dependent CFTR Macromolecular Signaling Complexes

Published on: August 13, 2012

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Cytoskeleton and CFTR.

Aleksander Edelman1

  • 1INSERM U1151, Université Paris Descartes, Paris, France.

The International Journal of Biochemistry & Cell Biology
|April 2, 2014
PubMed
Summary
This summary is machine-generated.

Cytoskeletal proteins interact with Cystic Fibrosis Transmembrane conductance Regulator (CFTR) to regulate ion transport, protein trafficking, and degradation. This interaction is crucial for understanding cystic fibrosis pathogenesis and potential therapies.

Keywords:
Cystic fibrosisIntermediary filamentsKeratin 8MicrofilamentsMicrotubules

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Functional Reconstitution and Channel Activity Measurements of Purified Wildtype and Mutant CFTR Protein
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Last Updated: May 1, 2026

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Functional Reconstitution and Channel Activity Measurements of Purified Wildtype and Mutant CFTR Protein
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Functional Reconstitution and Channel Activity Measurements of Purified Wildtype and Mutant CFTR Protein

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Physiology

Background:

  • Cystic Fibrosis Transmembrane conductance Regulator (CFTR) is a PKA-regulated Cl(-) channel vital for epithelial fluid transport.
  • Mutations in CFTR, particularly ΔF508, cause cystic fibrosis, a severe autosomal recessive disease.
  • CFTR regulation involves complex protein interactions, with emerging roles for cytoskeletal elements.

Purpose of the Study:

  • To review the dynamic interactions between cytoskeletal proteins (microfilaments, microtubules, intermediate filaments) and CFTR.
  • To elucidate the role of these interactions in regulating CFTR-dependent ion transport.
  • To explore the impact on CFTR trafficking and degradation pathways.

Main Methods:

  • Literature review focusing on molecular and cellular mechanisms.
  • Analysis of studies investigating protein-protein interactions.
  • Synthesis of current knowledge on cytoskeletal involvement in CFTR function.

Main Results:

  • Cytoskeletal proteins form a dynamic interactome with CFTR.
  • These interactions are critical for the regulation of transepithelial ion transport.
  • The cytoskeleton influences CFTR trafficking to and from the plasma membrane, as well as its degradation.

Conclusions:

  • Cytoskeletal networks are key regulators of CFTR function, trafficking, and stability.
  • Understanding these interactions offers new insights into cystic fibrosis.
  • Targeting cytoskeletal-CFTR interactions may present novel therapeutic strategies for cystic fibrosis.