Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Electronic Nicotine Delivery Systems and Clinical Outcomes in Adults With Inflammatory Bowel Disease: A Systematic Review of Emerging Evidence.

Cureus·2026
Same author

Vonoprazan vs Proton Pump Inhibitors for Gastroprotection in Anticoagulated Patients: A Real-World Cohort Study.

Cureus·2026
Same author

Synergistic Organic-Inorganic Interface Engineering for Stable Zinc Metal Anodes in Aqueous Batteries.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Comparative Outcomes of Arthroscopic Versus Open Meniscus Repair: An Observational Study.

Journal of orthopaedic case reports·2026
Same author

Retrograde Intramedullary Nailing Versus Locked Plating for Periprosthetic Distal Femur Fractures: A Propensity-matched Cohort from TriNetX.

Journal of orthopaedic case reports·2026
Same author

Trends in Rheumatoid Arthritis Mortality in the United States, 1999-2020.

Journal of orthopaedic case reports·2026

Related Experiment Video

Updated: May 1, 2026

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells
14:12

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells

Published on: December 11, 2021

5.1K

Using fluorescence correlation spectroscopy (FCS) for IFN-g detection: a preliminary study.

Chen Shen1, Martin Knapp2, Martin Gerald Puchinger2

  • 1Key Laboratory of Major Diseases in Children and National, Key Discipline of Pediatrics (Capital Medical University), Ministry of Education, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, Beijing 100045, China.

Journal of Immunological Methods
|April 2, 2014
PubMed
Summary

This study explores a new method for detecting interferon-gamma (IFN-g) using fluorescence correlation spectroscopy (FCS). This technique shows potential as an alternative to ELISA for diagnosing Mycobacterium tuberculosis infections.

Keywords:
DiagnosticFluorescence correlation spectroscopyInterferon-gammaMonoclonal antibodies

More Related Videos

A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts
08:43

A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts

Published on: December 1, 2018

10.5K
Determination of Lipid Raft Partitioning of Fluorescently-tagged Probes in Living Cells by Fluorescence Correlation Spectroscopy FCS
10:59

Determination of Lipid Raft Partitioning of Fluorescently-tagged Probes in Living Cells by Fluorescence Correlation Spectroscopy FCS

Published on: April 6, 2012

15.8K

Related Experiment Videos

Last Updated: May 1, 2026

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells
14:12

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells

Published on: December 11, 2021

5.1K
A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts
08:43

A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts

Published on: December 1, 2018

10.5K
Determination of Lipid Raft Partitioning of Fluorescently-tagged Probes in Living Cells by Fluorescence Correlation Spectroscopy FCS
10:59

Determination of Lipid Raft Partitioning of Fluorescently-tagged Probes in Living Cells by Fluorescence Correlation Spectroscopy FCS

Published on: April 6, 2012

15.8K

Area of Science:

  • Biotechnology
  • Immunology
  • Medical Diagnostics

Background:

  • Enzyme-linked immunosorbent assay (ELISA) is used to detect Mycobacterium tuberculosis (M. tuberculosis) antigen-stimulated interferon-gamma (IFN-g) secretion.
  • This method improves diagnostic value for M. tuberculosis infection.
  • Fluorescence correlation spectroscopy (FCS) has applications in medicine and molecular biology for biomarker detection.

Purpose of the Study:

  • To investigate the potential of fluorescence correlation spectroscopy (FCS) for detecting interferon-gamma (IFN-g).
  • To explore FCS as a novel diagnostic technique for M. tuberculosis infection.
  • To present preliminary findings on FCS-based IFN-g detection.

Main Methods:

  • Utilizing fluorescence correlation spectroscopy (FCS) for biomarker detection.
  • Focusing on the detection of interferon-gamma (IFN-g).
  • Comparing FCS technique with traditional ELISA methods.

Main Results:

  • Preliminary data suggests FCS can detect IFN-g.
  • The study presents initial findings on the feasibility of FCS for this application.
  • Further research is needed to validate FCS as a diagnostic tool.

Conclusions:

  • Fluorescence correlation spectroscopy (FCS) shows promise for detecting interferon-gamma (IFN-g).
  • FCS may offer an alternative to ELISA for diagnosing M. tuberculosis infection.
  • This preliminary study warrants further investigation into FCS-based diagnostics.