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Updated: May 1, 2026

Three-dimensional Optical-resolution Photoacoustic Microscopy
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Hybrid multiphoton and optoacoustic microscope.

George J Tserevelakis, Dominik Soliman, Murad Omar

    Optics Letters
    |April 2, 2014
    PubMed
    Summary

    This study introduces a hybrid microscope merging multiphoton microscopy and optoacoustic microscopy for advanced biological imaging. The combined system offers label-free visualization of zebrafish, enhancing multiparametric microscopy capabilities.

    Area of Science:

    • Biomedical Optics
    • Microscopy
    • Biophotonics

    Background:

    • Multiphoton microscopy (MPM) offers deep tissue imaging with intrinsic contrast.
    • Optical-resolution optoacoustic microscopy (OR-PAM) provides high-resolution structural information.
    • Combining complementary imaging modalities can enhance diagnostic and research capabilities.

    Purpose of the Study:

    • To develop and evaluate a hybrid microscope integrating MPM with second-harmonic generation (SHG) contrast and OR-PAM.
    • To assess the performance and contrast complementarity of the integrated system.
    • To demonstrate label-free multiparametric imaging of biological specimens.

    Main Methods:

    • A hybrid microscope system was constructed, combining MPM with SHG and OR-PAM.

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  • The system was tested on ex vivo zebrafish larvae.
  • Concurrent imaging of zebrafish musculature and melanocytes was performed.

    • Main Results:

    • The hybrid microscope successfully performed label-free imaging of zebrafish larvae.
    • Concurrent visualization of musculature and melanocytes was achieved, demonstrating contrast complementarity.
    • The system's performance was evaluated, highlighting its potential for multiparametric studies.

    Conclusions:

    • The hybrid MPM-SHG and OR-PAM microscope enables simultaneous, label-free imaging of biological structures.
    • This integrated approach enhances information acquisition from biological specimens.
    • The technology holds promise for advanced multiparametric microscopy in research and diagnostics.