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Related Experiment Video

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Trace Fear Conditioning in Mice
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Trace Fear Conditioning in Mice

Published on: March 20, 2014

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Trace fear conditioning in mice.

Joaquin N Lugo1, Gregory D Smith2, Andrew J Holley3

  • 1Department of Psychology and Neuroscience, Baylor University; Institute of Biomedical Studies, Baylor University; joaquin_lugo@baylor.edu.

Journal of Visualized Experiments : Jove
|April 2, 2014
PubMed
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This study introduces trace fear conditioning to measure learning and memory in mice. This method effectively detects subtle deficits and enhancements, offering insights into brain function.

Area of Science:

  • Neuroscience
  • Behavioral Science

Background:

  • Learning and memory are complex cognitive functions.
  • Fear conditioning is a widely used paradigm to study these processes.
  • Trace fear conditioning involves a temporal gap between conditioned and unconditioned stimuli, making it sensitive to certain memory deficits.

Purpose of the Study:

  • To present and validate a trace fear conditioning protocol for measuring learning and memory in mice.
  • To highlight the sensitivity of trace fear conditioning in detecting subtle deficits not identified by other methods.
  • To discuss the neural circuitry involved and controversies surrounding its amygdala independence.

Main Methods:

  • Utilized a trace fear conditioning protocol with five pairings of neutral and unconditioned stimuli.

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  • Incorporated a 20-second trace period between conditioning trials.
  • Measured freezing behavior during conditioned stimulus (CS) presentation and contextual memory tests.

    • Main Results:

    • Demonstrated successful application of trace fear conditioning in mice.
    • Showcased the ability of the method to detect learning and memory deficits and enhancements.
    • Presented representative results comparing mice receiving aversive stimuli versus control mice.

    Conclusions:

    • Trace fear conditioning is a valuable technique for assessing learning and memory, particularly for subtle alterations.
    • The protocol is believed to involve medial prefrontal cortex-hippocampus connections.
    • Further research, including delay fear conditioning, is necessary to explore amygdala-dependent learning and memory effects.