Detection and quantification of the Bcr/Abl chimeric protein on biochips using LDI-TOF MS
Seol-Hye Hong1, Jae Il Kim, Hyunook Kang
1Department of Bioscience and Biotechnology, Bio/Molecular Informatics Center, Konkuk University, Seoul 143-701, Korea. wsyeo@konkuk.ac.kr.
Abstract:
The Bcr/Abl chimeric protein was captured by two antibodies, anti-Bcr on gold nanoparticles (AuNPs) and anti-Abl on a biochip, in a sandwich assay format. The presence of the Bcr/Abl in cells was then verified by amplified LDI-TOF MS signals, and relative amounts were quantified using AuNPs coated with deuterated alkanethiols as an internal standard.


