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Updated: May 1, 2026

Determination of Chemical Inhibitor Efficiency against Intracellular Toxoplasma Gondii Growth Using a Luciferase-Based Growth Assay
Published on: April 29, 2020
Bumped kinase inhibitor 1294 treats established Toxoplasma gondii infection
J Stone Doggett1, Kayode K Ojo2, Erkang Fan3
1VA Medical Center, Portland, Oregon, USA Division of Infectious Diseases, Oregon Health & Science University, Portland, Oregon, USA doggettj@ohsu.edu wvanvoorhis@medicine.washington.edu.
Abstract:
Toxoplasma gondii is a unicellular parasite that causes severe brain and eye disease. Current drugs for T. gondii are limited by toxicity. Bumped kinase inhibitors (BKIs) selectively inhibit calcium-dependent protein kinases of the apicomplexan pathogens T. gondii, cryptosporidia, and plasmodia. A lead anti-Toxoplasma BKI, 1294, has been developed to be metabolically stable and orally bioavailable. Herein, we demonstrate the oral efficacy of 1294 against toxoplasmosis in vivo.
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