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Updated: May 1, 2026

Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay
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Global transcriptome analysis identifies regulated transcripts and pathways activated during oogenesis and early

Lene Kleppe1, Rolf Brudvik Edvardsen, Tomasz Furmanek

  • 1Institute of Marine Research, Bergen, Norway.

Molecular Reproduction and Development
|April 2, 2014
PubMed
Summary

This study reveals Atlantic cod

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Area of Science:

  • * Reproductive Biology
  • * Molecular Biology
  • * Marine Genomics

Background:

  • * Oogenesis and early embryogenesis in fish, particularly marine species, have poorly understood molecular mechanisms.
  • * Understanding maternal control over these processes is crucial for aquaculture and conservation efforts.

Purpose of the Study:

  • * To investigate the egg and embryo transcriptome during oogenesis and early embryogenesis in Atlantic cod.
  • * To identify key genes and pathways involved in maternal transcript regulation and zygotic genome activation.

Main Methods:

  • * Transcriptome profiling using a 44K oligo microarray with 23,000 Atlantic cod genes.
  • * Analysis of gene expression in ovarian follicles (pre-, early-, and late-vitellogenic), ovulated eggs, and embryonic stages (blastula, gastrula).

Main Results:

  • * Hundreds of differentially expressed genes (DEGs) were identified throughout oogenesis, indicating dynamic transcript accumulation and degradation.
  • * A stable maternal RNA pool was observed from ovulated egg to blastula stage.
  • * Significant zygotic transcription onset was detected between blastula and gastrula stages, with upregulated genes linked to nervous system signaling, metabolism, and energy production.

Conclusions:

  • * This study provides a comprehensive overview of the Atlantic cod's maternal transcriptome from oogenesis to early embryogenesis.
  • * Identified gene expression patterns highlight critical molecular events, including synaptic signaling, metabolic adjustments, and the initiation of embryonic development.