Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Interferon induction under in vitro conditions].

B Glück1, A Stelzner

  • 1Akademie der Wissenschaften der DDR, Zentral-institut für Mikrobiologie und experimentelle Therapie, Jena.

Journal of Basic Microbiology
|January 1, 1989
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Tungsten carbide nanoparticles show a broad spectrum virucidal activity against enveloped and nonenveloped model viruses using a guideline-standardized in vitro test.

Letters in applied microbiology·2019
Same author

Preselection of Potential Cancerostatics by Automatic Analysis of Suspended and Adherent Cells Incubated in Microplates.

Alternatives to laboratory animals : ATLA·2015
Same author

Search for photon-linelike signatures from dark matter annihilations with H.E.S.S.

Physical review letters·2014
Same author

Search for a dark matter annihilation signal from the galactic center halo with H.E.S.S.

Physical review letters·2011
Same author

Localization of intra-ocular foreign bodies.

The British journal of ophthalmology·2010
Same author

Detection of gamma rays from a starburst galaxy.

Science (New York, N.Y.)·2009
Same journal

Biofertilizers.

Journal of basic microbiology·2026
Same journal

Synergistic Biodegradation of Monocrotophos and Plant Growth Stimulation by Soil Bacterial Strains.

Journal of basic microbiology·2026
Same journal

Microbial Shifts Across Endosphere and Rhizosphere as Strategy for Drought Adaptation in Shrub Ammopiptanthus mongolicus.

Journal of basic microbiology·2026
Same journal

Using Trichoderma spp. to Biological Control of Rhizoctonia solani Root Rot in Faba Beans.

Journal of basic microbiology·2026
Same journal

A One-Step Dual Chromogenic Plate Assay for Reliable Screening of Naringinase-Producing Microorganisms.

Journal of basic microbiology·2026
Same journal

Arbuscular Mycorrhizal Fungal Diversity in Taxus contorta Griff. Assessed Through Morphology and Amplicon Sequencing.

Journal of basic microbiology·2026
See all related articles

Optimizing interferon (IFN) production requires specific conditions. This study found optimal induction at 37°C in human fibroblasts using Poly (IC), with dose-dependent responses and enhanced induction via priming and metabolic inhibitors.

Area of Science:

  • Immunology and Virology
  • Cell Biology and Molecular Genetics

Background:

  • Standard interferon (IFN) induction methods utilize fibroblast or leukocyte cultures.
  • Understanding factors influencing IFN production capacity is crucial for optimizing antiviral therapies.

Purpose of the Study:

  • To investigate key factors affecting in vitro interferon induction efficiency.
  • To compare the inducer sensitivity of different human cell types.
  • To identify optimal conditions for maximizing interferon production.

Main Methods:

  • Induction of human fibroblasts and leukocytes with Poly (IC) under varying temperatures (30°C vs. 37°C).
  • Dose-response studies using different concentrations of the inducing agent.
  • Assessment of super-induction using metabolic inhibitors (actinomycin D, cycloheximide).

Related Experiment Videos

  • Evaluation of priming procedures to enhance IFN production.
  • Comparative analysis of interferon induction across diverse cell types and culture systems.

    • Main Results:

    • Optimal interferon induction in human fibroblasts occurred at 37°C, contrary to previous findings.
    • A direct correlation was observed between the dose of the inducer (Poly (IC)) and the amount of IFN produced.
    • Cells demonstrated significant super-inducibility with metabolic inhibitors and enhanced IFN production following priming.
    • Substantial variability in inducer sensitivity was noted among different cell types and culture systems.

    Conclusions:

    • Temperature, inducer dose, metabolic inhibitors, and priming significantly influence in vitro interferon induction.
    • Human fibroblast interferon induction is optimized at 37°C with Poly (IC).
    • Cell type and culture system choice critically impact interferon production efficiency.