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Plasma 5-hydroxyindoleacetic acid as an endogenous index of renal plasma flow
T Hannedouche1, D Laude, M Déchaux
1Department of Nephrology, Hôpital Necker-Enfants Malades, Paris, France.
Abstract:
5-Hydroxyindoleacetic acid (5-HIAA), the major serotonin metabolite, was measured in human plasma and urine using liquid chromatography and electrochemical detection. The renal extraction of 5-HIAA was 78%, a value compatible with an almost complete extraction during a single passage of the blood through the kidney. In addition, plasma levels of 5-HIAA measured in patients with a wide range of renal function was inversely correlated (r = 0.85) with the clearance of para-aminohippuric acid (PAH). The results indicate that 5-HIAA determinations can be used as an alternative procedure to the PAH clearance method for the estimation of renal plasma flow in clinical practice.