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Next generation sequencing: potential and application in drug discovery.
Navneet Kumar Yadav1, Pooja Shukla2, Ankur Omer2
1Hematological Facility, Division of Toxicology, CSIR-Central Drug Research Institute, BS-10/1, Sector 10, Jankipuram Extension, Sitapur Road, P.O. Box 173, Lucknow 226031, India.
Thescientificworldjournal
|April 2, 2014
Summary
Next-generation sequencing (NGS) technologies are revolutionizing genomics, impacting plant and animal research. These advanced methods promise to surpass Sanger sequencing and accelerate drug discovery.
Area of Science:
- Genomics
- Molecular Biology
- Bioinformatics
Background:
- The field of genomics has entered a new era driven by high-throughput sequencing.
- Traditional Sanger sequencing has been the standard for three decades.
- Advancements in sequencing technologies are crucial for biological research.
Purpose of the Study:
- To highlight the impact of next-generation sequencing (NGS) technologies.
- To discuss the potential of NGS in addressing genomic challenges in plants and animals.
- To explore the role of NGS in accelerating drug discovery.
Main Methods:
- Next-generation sequencing (NGS) technologies.
- Includes sequencing by synthesis-fluorescent in situ sequencing (FISSEQ), pyrosequencing, sequencing by ligation, supported oligonucleotide detection (SOLiD), sequencing by hybridization, and nanopore technology.
- Comparison with Sanger sequencing.
Main Results:
- NGS technologies offer significant advancements over previous methods.
- These technologies are instrumental in solving complex genome-related problems in the plant and animal kingdoms.
- NGS is poised to supersede Sanger sequencing.
Conclusions:
- NGS heralds a new era in genomics research.
- The application of NGS will broaden our understanding of plant and animal genomes.
- NGS is expected to significantly expedite the drug discovery process.
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