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Computer-Aided Protein Directed Evolution: a Review of Web Servers, Databases and other Computational Tools for
Rajni Verma1, Ulrich Schwaneberg2, Danilo Roccatano3
1School of Engineering and Science, Jacobs University Bremen, Campus Ring 1, 28759 Bremen, Germany ; Department of Biotechnology, RWTH Aachen University, Worringer Weg 1, 52074 Aachen, Germany.
Computational and Structural Biotechnology Journal
|April 2, 2014
Summary
Computer-aided protein directed evolution (CAPDE) combines computational and directed evolution for protein engineering. This review covers recent computational tools to aid CAPDE experiments.
Area of Science:
- Biochemistry
- Bioinformatics
- Protein Engineering
Background:
- Protein engineering is crucial for developing novel enzymes and therapeutics.
- Directed evolution is a powerful method for protein optimization.
- Integrating computational approaches enhances directed evolution efficiency.
Purpose of the Study:
- To review recent computational tools for protein engineering.
- To assess the availability, usability, and limitations of these resources.
- To guide the design of computer-aided protein directed evolution (CAPDE) experiments.
Main Methods:
- Literature review of computational tools and resources.
- Focus on web servers, databases, and software developed in the last five years.
- Analysis of tool accessibility and practical application in protein engineering.
Main Results:
- Identification of key computational resources for CAPDE.
- Evaluation of the strengths and weaknesses of current tools.
- Summary of trends in computational tool development for protein engineering.
Conclusions:
- CAPDE is a rapidly advancing field.
- Accessible and user-friendly computational tools are essential for advancing protein engineering.
- This review provides a concise guide to current resources for researchers.
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