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Related Concept Videos

Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

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Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
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Mass Spectrometry: Complex Analysis01:21

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Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
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Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
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Updated: May 1, 2026

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS
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Integrated LC-MS/MS system for plant metabolomics.

Yuji Sawada1, Masami Yokota Hirai2

  • 1RIKEN Center for Sustainable Resource Science, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan ; RIKEN Plant Science Center, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan.

Computational and Structural Biotechnology Journal
|April 2, 2014
PubMed
Summary

Liquid chromatography-tandem mass spectrometry (LC-MS/MS) enables broad metabolite detection in plants. Integrated omics analyses aid in identifying unknown metabolites, advancing plant metabolomics research.

Keywords:
MS/MSQ-TOF-MSTQ-MSquantitative trait locus analysisselected reaction monitoring

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Area of Science:

  • Plant biology
  • Metabolomics
  • Analytical chemistry

Background:

  • Liquid chromatography-tandem mass spectrometry (LC-MS/MS) offers high sensitivity and selectivity for metabolite detection.
  • Comprehensive metabolite profiling is crucial for understanding plant biochemistry.
  • Identifying unknown metabolites remains a significant challenge in untargeted metabolomics.

Purpose of the Study:

  • To demonstrate the utility of integrated LC-MS/MS and omics analyses for plant metabolomics.
  • To characterize metabolite accumulation patterns without relying on standard compounds.
  • To address the challenge of identifying unknown metabolites in plant samples.

Main Methods:

  • Utilizing liquid chromatography-tandem mass spectrometry (LC-MS/MS) for untargeted plant metabolomics.
  • Employing integrated omics analyses, including reverse genetics, linkage mapping, and association mapping.
  • Leveraging reference database searches for structural assignment of detected metabolites.

Main Results:

  • LC-MS/MS detected several hundred metabolites in plant samples.
  • Integrated omics analyses provided a powerful tool for candidate structure selection and rejection.
  • The study facilitated the characterization of a large number of detectable metabolites.

Conclusions:

  • Integrated LC-MS/MS and omics analyses are effective for comprehensive plant metabolomics.
  • This approach enables metabolite characterization without the need for isolated compounds or standards.
  • Emerging technologies enhance the capabilities of untargeted plant metabolomics for metabolite discovery.