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Related Experiment Video

Updated: May 1, 2026

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
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Tos17 rice element: incomplete but effective.

Francois Sabot1

  • 1UMR DIADE IRD/UM2, 911 Avenue Agropolis BP64503, F-34394 Montpellier Cedex 5, France. francois.sabot@ird.fr.

Mobile DNA
|April 3, 2014
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Summary
This summary is machine-generated.

Tos17, a rice retrotransposon, was analyzed. Unexpectedly, it lacks essential sequences, indicating it cannot move independently and requires helper elements for transposition.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Plant Science

Background:

  • Tos17 is the first described active LTR retrotransposon (Copia) in cultivated rice.
  • It exists in two copies in the Nipponbare rice genome, with only the chromosome 7 copy showing activity.
  • Its retrotransposition ability has been utilized in insertional mutagenesis.

Purpose of the Study:

  • To thoroughly annotate the structure of the active Tos17 retrotransposon.
  • To elucidate the molecular mechanisms underlying Tos17 activity and its potential for autonomous transposition.

Main Methods:

  • Bioinformatic analyses were employed to annotate the Tos17 structure.
  • Comparative genomics and sequence analysis were utilized to identify key genetic elements.

Main Results:

  • The detailed annotation revealed specific structural features of the active Tos17 copy.
  • Bioinformatic analysis identified the absence of the gag sequence within the Tos17 structure.

Conclusions:

  • Tos17 is unexpectedly characterized as a non-autonomous LTR retrotransposon.
  • The lack of the gag sequence prevents Tos17 from self-transposition, suggesting a dependence on other genetic elements.