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Related Experiment Video

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A Semi-automated Approach to Preparing Antibody Cocktails for Immunophenotypic Analysis of Human Peripheral Blood
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Immunophenotyping of paucicellular samples.

Alessandra Stacchini1, Anna Demurtas1, Sabrina Aliberti1

  • 1Flow Cytometry Unit, Anatomical Pathology, Diagnostic Laboratory Department, Città della Salute e della Scienza, Turin, Italy.

Current Protocols in Cytometry
|April 3, 2014
PubMed
Summary
This summary is machine-generated.

This study presents a flow cytometry (FC) method for analyzing low-cell samples like fine needle aspirates (FNA) and body fluids (BF). The technique maximizes diagnostic information from limited cell populations, aiding lymphoma detection and monitoring.

Keywords:
multiparameter flow cytometrynon-Hodgkin lymphomaspaucicellular samples

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Area of Science:

  • Clinical laboratory science
  • Immunology
  • Hematology

Background:

  • Paucicellular samples pose diagnostic challenges in routine flow cytometry (FC).
  • Low cell counts in samples like fine needle aspirates (FNA), body fluids (BF), cerebrospinal fluid (CSF), and ocular fluid (OF) limit testing capabilities.
  • Accurate immunophenotyping is crucial for lymphoma diagnosis and monitoring.

Purpose of the Study:

  • To develop a method for maximizing diagnostic yield from paucicellular samples in FC.
  • To enable comprehensive lymphocyte population analysis and aberrant cell detection from limited cellular material.
  • To address the challenges of immunophenotyping rare cell populations.

Main Methods:

  • Utilizes a combination of multiple monoclonal antibodies (MAbs).
  • Employs a Boolean gating strategy for data analysis.
  • Focuses on a single-tube approach to conserve precious cellular material.

Main Results:

  • Successfully identifies and quantifies major lymphocyte populations.
  • Enables detection of lymphomatous B cells.
  • Facilitates identification of aberrant T cell expression in paucicellular samples.

Conclusions:

  • The described protocol effectively extracts maximum diagnostic information from low-cell samples.
  • This method enhances the utility of FC for diagnosing and monitoring lymphoid malignancies in challenging specimens.
  • The single-tube approach with Boolean gating is vital for efficient analysis of paucicellular samples.