Jove
Visualize
Contact Us

Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

1.9K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
1.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

iSCORE-PD: an isogenic stem cell collection to research Parkinson's disease.

Nature communications·2026
Same author

Alternative pre-mRNA Splicing and Gene Expression Patterns in Midbrain Lineage Cells Carrying Familial Parkinson's Disease Mutations.

bioRxiv : the preprint server for biology·2025
Same author

Activity of zebrafish THAP9 transposase and zebrafish P element-like transposons.

bioRxiv : the preprint server for biology·2024
Same author

iSCORE-PD: an isogenic stem cell collection to research Parkinson's Disease.

bioRxiv : the preprint server for biology·2024
Same author

A mutation in the low-complexity domain of splicing factor hnRNPA1 linked to amyotrophic lateral sclerosis disrupts distinct neuronal RNA splicing networks.

Genes & development·2024
Same author

Highly efficient generation of isogenic pluripotent stem cell models using prime editing.

eLife·2022
Same journal

High-Throughput Microbial Assay for Amino Acid Measurement in Ground Maize Seed Samples Utilizing Auxotrophic <i>E. coli</i>.

Cold Spring Harbor protocols·2025
Same journal

Grain Quality in Maize.

Cold Spring Harbor protocols·2025
Same journal

High-Throughput Assay for Measuring Phytate and Available Phosphorus in Ground Maize Seed Samples.

Cold Spring Harbor protocols·2025
Same journal

Functional Genomic Analysis of Transposon Insertion Mutant Maize Plants from the UniformMu National Public Resource.

Cold Spring Harbor protocols·2025
Same journal

The UniformMu National Public Resource: Transposon<i>-</i>Induced Mutant Seeds for Functional Genomics Studies in Maize.

Cold Spring Harbor protocols·2025
Same journal

Insights from the Study of B<i>-</i>Cell Epitopes of a Microbial Pathogen by Phage Display.

Cold Spring Harbor protocols·2025
See all related articles
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 1, 2026

Electrophoretic Mobility Shift Assay EMSA for the Study of RNA-Protein Interactions: The IRE/IRP Example
12:44

Electrophoretic Mobility Shift Assay EMSA for the Study of RNA-Protein Interactions: The IRE/IRP Example

Published on: December 3, 2014

53.4K

Electrophoretic mobility shift assays for RNA-protein complexes.

Donald C Rio

    Cold Spring Harbor Protocols
    |April 3, 2014
    PubMed
    Summary
    This summary is machine-generated.

    The electrophoretic mobility shift assay (EMSA) detects RNA-protein interactions by observing changes in RNA mobility on a gel. This powerful technique is valuable for studying complex biological interactions and determining binding affinities.

    More Related Videos

    An Optimized Protocol for Electrophoretic Mobility Shift Assay Using Infrared Fluorescent Dye-labeled Oligonucleotides
    09:58

    An Optimized Protocol for Electrophoretic Mobility Shift Assay Using Infrared Fluorescent Dye-labeled Oligonucleotides

    Published on: November 29, 2016

    15.3K
    Horizontal Gel Electrophoresis for Enhanced Detection of Protein-RNA Complexes
    06:36

    Horizontal Gel Electrophoresis for Enhanced Detection of Protein-RNA Complexes

    Published on: July 28, 2017

    11.2K

    Related Experiment Videos

    Last Updated: May 1, 2026

    Electrophoretic Mobility Shift Assay EMSA for the Study of RNA-Protein Interactions: The IRE/IRP Example
    12:44

    Electrophoretic Mobility Shift Assay EMSA for the Study of RNA-Protein Interactions: The IRE/IRP Example

    Published on: December 3, 2014

    53.4K
    An Optimized Protocol for Electrophoretic Mobility Shift Assay Using Infrared Fluorescent Dye-labeled Oligonucleotides
    09:58

    An Optimized Protocol for Electrophoretic Mobility Shift Assay Using Infrared Fluorescent Dye-labeled Oligonucleotides

    Published on: November 29, 2016

    15.3K
    Horizontal Gel Electrophoresis for Enhanced Detection of Protein-RNA Complexes
    06:36

    Horizontal Gel Electrophoresis for Enhanced Detection of Protein-RNA Complexes

    Published on: July 28, 2017

    11.2K

    Area of Science:

    • Molecular Biology
    • Biochemistry
    • Biophysics

    Background:

    • RNA-protein interactions are fundamental to numerous cellular processes.
    • Detecting and characterizing these interactions is crucial for understanding gene regulation and cellular function.
    • Existing methods may have limitations in sensitivity or applicability to complex biological samples.

    Purpose of the Study:

    • To describe the principles and applications of the electrophoretic mobility shift assay (EMSA).
    • To highlight EMSA's utility in studying RNA-protein binding.
    • To demonstrate EMSA's capability in analyzing complex biological mixtures and determining binding kinetics.

    Main Methods:

    • Electrophoretic separation of radiolabeled RNA on nondenaturing gels.
    • Monitoring the mobility shift of RNA upon protein binding.
    • Utilizing complex biological mixtures, such as cell extracts, for interaction studies.
    • Analyzing shifts to determine binding affinity and kinetics.

    Main Results:

    • EMSA effectively detects RNA-protein interactions by reduced RNA mobility when bound to proteins.
    • The assay allows for the identification of specific, high-affinity interactions within complex mixtures.
    • EMSA can resolve single binding events up to large complex assemblies like the spliceosome.
    • Kinetic parameters, including affinity constants, can be determined.

    Conclusions:

    • EMSA is a robust and versatile technique for investigating RNA-protein interactions.
    • Its ability to handle complex samples and quantify binding makes it invaluable in molecular biology research.
    • EMSA facilitates the study of a wide spectrum of RNA-protein binding phenomena, from simple to intricate.