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Induction of apoptosis by deinoxanthin in human cancer cells

Yong-Ji Choi1, Jung-Mu Hur, Sangyong Lim

  • 1Department of Biotechnology and Bioengineering, Chonnam National University, Gwangju 500-757, South Korea. choiji01@jnu.ac.kr.

Anticancer Research
|April 3, 2014
PubMed
Abstract

Insights

Deinoxanthin, a carotenoid from Deinococcus radiodurans, effectively induces apoptosis in cancer cells by increasing reactive oxygen species (ROS) and altering apoptotic protein expression. This suggests its potential as a chemopreventive agent.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Microbiology

Background:

  • Deinoxanthin is a unique carotenoid derived from the radioresistant bacterium Deinococcus radiodurans.
  • Its potential role in inducing cancer cell apoptosis has not been previously explored.

Purpose of the Study:

  • To investigate the apoptosis-inducing effects of deinoxanthin on various cancer cell lines.
  • To elucidate the underlying mechanisms, including reactive oxygen species (ROS) generation and apoptotic protein modulation.

Main Methods:

  • Evaluated apoptotic effects in HepG2, PC-3, and HT-29 cells using assays for cell viability, morphology, and DNA fragmentation.
  • Measured intracellular ROS generation using carboxy-H2DCF-DA.
  • Assayed apoptotic and anti-apoptotic protein expression via western blotting.

Main Results:

  • Deinoxanthin exhibited half-maximal inhibitory concentrations (IC50) of 59 μM (HepG2), 61 μM (HT-29), and 77 μM (PC-3).
  • Treatment increased ROS levels in all tested cancer cells, indicating potential pro-oxidant activity.
  • Deinoxanthin treatment led to pro-caspase-3 degradation and altered BCL2/BAX expression ratios, favoring apoptosis.

Conclusions:

  • This study demonstrates deinoxanthin's novel function as a potent inducer of apoptosis in cancer cells.
  • The findings suggest deinoxanthin holds promise as a potential chemopreventive agent.
Keywords:
Deinoxanthinapoptosiscancer cellsreactive oxygen species

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