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Related Experiment Video

Updated: May 1, 2026

Characterization of Amyloid Structures in Aging C. Elegans Using Fluorescence Lifetime Imaging

Characterization of Amyloid Structures in Aging C. Elegans Using Fluorescence Lifetime Imaging

Published on: March 27, 2020

PML-nuclear bodies decrease with age and their stress response is impaired in aged individuals.

Barbara Wenger, Manuela Schwegler, Maria Brunner

¹Department of Radiation Oncology, University Hospitals and Friedrich-Alexander-University Erlangen-Nürnberg, Universitätsstraße 27, D-91054 Erlangen, Germany. Luitpold.Distel@uk-erlangen.de.

|April 4, 2014

Summary

This summary is machine-generated.

Promyelocytic leukemia-nuclear bodies (PML-NBs) decline with age and show a dysfunctional stress response in older individuals. This study investigated PML-NBs in aging, cancer, and radiation exposure.

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Characterization of Amyloid Structures in Aging C. Elegans Using Fluorescence Lifetime Imaging

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Published on: March 27, 2020

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Detection of Nuclear Blebbing and DNA Leakage in Mammalian Cells by Immunofluorescence

Detection of Nuclear Blebbing and DNA Leakage in Mammalian Cells by Immunofluorescence

Published on: January 17, 2025

Area of Science:

Cellular biology
Aging research
Cancer biology

Background:

Promyelocytic leukemia-nuclear bodies (PML-NBs) are involved in DNA damage repair and stress response.
Cellular aging affects DNA repair mechanisms and stress resilience.
Understanding PML-NBs' role in aging is crucial for age-related disease research.

Purpose of the Study:

To evaluate age-dependent differences in PML-NBs response to stress.
To investigate the impact of cancer and ionizing radiation on PML-NBs across different age groups.
To clarify the role of PML-NBs in the aging process.

Main Methods:

Examined peripheral blood monocytes from 134 cancer patients and 41 healthy individuals (ages 22-92).
Analyzed cells before and after in vitro irradiation, and cancer patients' samples after in vivo irradiation.
Quantified PML-NBs and γH2AX foci using immunostaining in approximately 1600 cells per individual.

Main Results:

PML-NBs count per nucleus decreased with age.
γH2AX foci count increased with age, indicating elevated DNA damage.
In vivo irradiation showed a trend of increasing PML-NBs in younger individuals and decreasing them in older individuals.

Conclusions:

PML-NBs exhibit a reduced and potentially dysfunctional stress response in aged cells.
Aging is associated with decreased PML-NBs and impaired DNA damage response.
Findings suggest PML-NBs play a significant role in cellular aging and stress resilience.