Related Experiment Video
Updated: May 1, 2026
10:00
Multi-Stream Perfusion Bioreactor Integrated with Outlet Fractionation for Dynamic Cell Culture
Published on: July 20, 2022
2.2K
Microfluidic perfusion cell culture system confined in 35 mm culture dish for standard biological laboratories
Eitaro Kondo1, Ken-Ichi Wada2, Kazuo Hosokawa2
1Bioengineering Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan; Department of Advanced Materials Science, University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba 227-8561, Japan.
Journal of Bioscience and Bioengineering
|April 4, 2014
Abstract:
An extremely simple, self-standing microfluidic cell culture system is reported. The whole system is confined in a 35 mm culture dish, and requires only a standard CO2 incubator. The culture medium is perfused by gravity. We successfully cultured NIH3T3-derived cells up to 10 days with a viability of ∼90%.
