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Related Experiment Videos

High pp60c-src level in human platelet dense bodies.

F Rendu1, M Lebret, S Danielian

  • 1U150 INSERM, URA 184 CNRS.

Blood
|May 1, 1989
PubMed
Summary
This summary is machine-generated.

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Redistribution of granulophysin and SRC protein in normal and gray platelets after activation.

Platelets·2010

Platelet activation by thrombin increases phosphotyrosine proteins, notably pp60c-src. This protein is concentrated in dense granules, suggesting its role in early platelet activation.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Hematology

Background:

  • Platelets play a crucial role in hemostasis and thrombosis.
  • Protein phosphorylation is a key regulatory mechanism in cellular signaling.
  • The Src family kinases are involved in various cellular processes, including cell growth and differentiation.

Purpose of the Study:

  • To investigate the changes in phosphoproteins during platelet activation.
  • To identify and characterize the specific tyrosine kinase involved in thrombin-induced platelet activation.
  • To determine the subcellular localization of this kinase within human platelets.

Main Methods:

  • In vivo labeling of phosphoproteins in resting and activated human platelets.
  • Analysis of phosphotyrosine-containing proteins using SDS-PAGE and electroblotting.

Related Experiment Videos

  • Identification of proteins using immunoprecipitation and electrotransfer techniques.
  • Subcellular fractionation to isolate platelet components like membranes, alpha-granules, lysosomes, and dense granules.

    • Main Results:

    • Thrombin stimulation led to an increase in phosphotyrosine-labeled proteins.
    • A prominent 60 kDa protein was identified as pp60c-src, the cellular homolog of the Rous sarcoma virus transforming protein.
    • pp60c-src was found to be highly enriched in platelet dense granules.
    • Other cellular compartments showed lower concentrations of pp60c-src.

    Conclusions:

    • Activated pp60c-src is a significant phosphoprotein in thrombin-stimulated platelets.
    • The enrichment of pp60c-src in dense granules suggests a specific role in granule-mediated platelet functions.
    • Protein phosphorylation mediated by pp60c-src is likely involved in the early stages of platelet activation.